PMID- 12597359
OWN - NLM
STAT- MEDLINE
DCOM- 20030411
LR  - 20190822
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 46
IP  - 12
DP  - 2002
TI  - Construction and in vitro properties of chimeric simian and human 
      immunodeficiency virus with the human TNF-alpha gene.
PG  - 849-55
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been reported to be involved in the 
      development and progression of acquired immunodeficiency syndrome (AIDS). To 
      study the role of this cytokine in AIDS pathogenesis, we constructed a chimeric 
      simian and human immunodeficiency virus (SHIV) having the human TNF-alpha gene 
      (SHIV-TNF) and characterized its properties in vitro. SHIV-TNF replicated both in 
      M8166, a human T cell line, and in monkey peripheral blood mononuclear cells 
      (PBMCs). Along with SHIV-TNF replication, TNF-alpha was detected in the culture 
      supernatant by ELISA. The maximum expression level of TNF-alpha reached 120 ng/ml 
      in M8166 cells, and 2.5 ng/ml in monkey PBMCs. The expressed TNF was biologically 
      active, as shown by a cytotoxic assay using TNF-sensitive L929 mouse fibroblasts. 
      This activity was detected at least until 10 passages of SHIV-TNF (74 days after 
      the initial infection). In monkey PBMCs, SHIV-TNF replicated much better than the 
      parental SHIV-NI. Flow cytometric analysis showed that the death of monkey PBMCs 
      infected with SHIV-TNF was severer than that caused by the parental SHIV-NI. 
      These results suggest that SHIV-TNF would be useful for inducing the disease in a 
      monkey model, which may contribute to a better understanding of the role of 
      TNF-alpha in AIDS etiology.
FAU - Haga, Takeshi
AU  - Haga T
AD  - Department of Veterinary Microbiology, Miyazaki University, Kibanadai Nishi, 
      Miyazaki 889-2192, Japan. a0d518u@cc.miyazaki-u.ac.jp
FAU - Shimizu, Yuya
AU  - Shimizu Y
FAU - Okoba, Masashi
AU  - Okoba M
FAU - Kumabe, Shino
AU  - Kumabe S
FAU - Goto, Yoshitaka
AU  - Goto Y
FAU - Shinjo, Toshiharu
AU  - Shinjo T
FAU - Ichimura, Hiroshi
AU  - Ichimura H
FAU - Kuwata, Takeo
AU  - Kuwata T
FAU - Hayami, Masanori
AU  - Hayami M
FAU - Miura, Tomoyuki
AU  - Miura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Annexin A5)
RN  - 0 (DNA, Recombinant)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Annexin A5/analysis
MH  - Cell Line
MH  - DNA, Recombinant/genetics
MH  - HIV/*genetics
MH  - Humans
MH  - Macaca
MH  - Models, Genetic
MH  - Recombination, Genetic
MH  - Simian Immunodeficiency Virus/*genetics
MH  - Tumor Necrosis Factor-alpha/analysis/*genetics
EDAT- 2003/02/25 04:00
MHDA- 2003/04/12 05:00
CRDT- 2003/02/25 04:00
PHST- 2003/02/25 04:00 [pubmed]
PHST- 2003/04/12 05:00 [medline]
PHST- 2003/02/25 04:00 [entrez]
AID - 10.1111/j.1348-0421.2002.tb02772.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2002;46(12):849-55. doi: 10.1111/j.1348-0421.2002.tb02772.x.