PMID- 12598424
OWN - NLM
STAT- MEDLINE
DCOM- 20030827
LR  - 20181113
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 138
IP  - 4
DP  - 2003 Feb
TI  - CC chemokines induce P-selectin-dependent neutrophil rolling and recruitment in 
      vivo: intermediary role of mast cells.
PG  - 698-706
AB  - 1. Based on in vitro chemotaxis experiments, it is widely held that CC 
      chemokines, such as macrophage inflammatory protein-1alpha (MIP-1alpha) and 
      macrophage chemotactic protein-1 (MCP-1) mainly support lymphocyte trafficking. 
      2. The objective of the present study was to examine the role of MIP-1alpha and 
      MCP-1 in neutrophil recruitment in vivo by use of intravital microscopy of the 
      mouse cremaster microcirculation. 3. MIP-1alpha and MCP-1 caused a dose-dependent 
      increase in leukocyte rolling, adhesion and recruitment. Indeed, neutrophils 
      comprised more than 85% of the leukocyte response to MIP-1alpha and MCP-1. An 
      anti-P-selectin antibody reduced MIP-1alpha and MCP-1-provoked leukocyte rolling 
      by more than 94%. Concomitantly, firm adhesion and extravasation of neutrophils 
      in response to MIP-1alpha and MCP-1 challenge were significantly decreased by 
      more than 78 and 84%, respectively. In contrast, an anti-E-selectin antibody had 
      no influence on CC chemokine-induced neutrophil recruitment. 4. Flow cytometric 
      analysis revealed that MIP-1alpha and MCP-1 had no effect on P-selectin 
      expression on endothelial cells, suggesting that neutrophil recruitment elicited 
      by CC chemokines in vivo is not mediated via a direct effect on the endothelium 
      but rather via an indirect effect involving activation of an intermediary tissue 
      cell. Indeed, it was found that MIP-1alpha-induced neutrophil accumulation was 
      significantly decreased by 58% in mast cell-deficient mice. 5. These findings 
      demonstrate that CC chemokines trigger P-selectin-dependent rolling and tissue 
      recruitment of neutrophils via tissue mast cells in vivo and suggest that CC 
      chemokines may also be important targets in neutrophil-mediated tissue damage in 
      multicellular organs.
FAU - Wan, Ming Xiu
AU  - Wan MX
AD  - Department of Surgery, Malmo University Hospital, Lund University, 20502 Malmo, 
      Sweden.
FAU - Wang, Yusheng
AU  - Wang Y
FAU - Liu, Qing
AU  - Liu Q
FAU - Schramm, Rene
AU  - Schramm R
FAU - Thorlacius, Henrik
AU  - Thorlacius H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Chemokines, CC)
RN  - 0 (P-Selectin)
SB  - IM
MH  - Animals
MH  - Chemokines, CC/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neutrophil Infiltration/*drug effects/physiology
MH  - Neutrophils/drug effects/metabolism
MH  - P-Selectin/*metabolism
PMC - PMC1573702
EDAT- 2003/02/25 04:00
MHDA- 2003/08/28 05:00
PMCR- 2004/02/01
CRDT- 2003/02/25 04:00
PHST- 2003/02/25 04:00 [pubmed]
PHST- 2003/08/28 05:00 [medline]
PHST- 2003/02/25 04:00 [entrez]
PHST- 2004/02/01 00:00 [pmc-release]
AID - 0705094 [pii]
AID - 10.1038/sj.bjp.0705094 [doi]
PST - ppublish
SO  - Br J Pharmacol. 2003 Feb;138(4):698-706. doi: 10.1038/sj.bjp.0705094.