PMID- 12600742
OWN - NLM
STAT- MEDLINE
DCOM- 20031027
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 86
IP  - 1
DP  - 2003 Mar 3
TI  - Effect of BCG vaccination on cytokine mRNA expression in atopic children with 
      asthma.
PG  - 29-35
AB  - BACKGROUND: To investigate whether a preexisting T(H2)-type immune response could 
      be suppressed by BCG immunization in atopic children with asthma. METHODS AND 
      RESULTS: We have used PCR to amplify reverse transcribed (RT) IFN-gamma and IL-5 
      mRNA expressed by peripheral blood mononuclear cells (PBMCs) in response to in 
      vitro phytohemagglutinin A, purified protein derivative and Dermatophagoides 
      pteronyssinus II stimulation from nine atopic children, both before and 8 weeks 
      after BCG vaccination. We have demonstrated that IFN-gamma expression was induced 
      in response to all stimulants (IFN-gamma/beta-actin) after the vaccination, 
      whereas there was no expression before (P<0.001). Although there was a tendency 
      to diminish in the expression of IL-5 mRNA in response to the stimulants, only 
      PHA rendered a statistically significant decrease after the vaccination. 
      CONCLUSIONS: These results provide some evidence of TH1 dominance after BCG 
      administration in atopic children.
FAU - Ozer, Ayse
AU  - Ozer A
AD  - Department of Medical Biology, School of Medicine, Marmara University, 
      Haydarpasa, 81326 Istanbul, Turkey. aozer@superonline.com
FAU - Tukenmez, Faruk
AU  - Tukenmez F
FAU - Biricik, Anil
AU  - Biricik A
FAU - Barlan, Isil B
AU  - Barlan IB
FAU - Cirakoglu, Beyazit
AU  - Cirakoglu B
FAU - Basaran, Mujdat M
AU  - Basaran MM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (BCG Vaccine)
RN  - 0 (Cytokines)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tuberculin)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/pharmacology
MH  - Asthma/*drug therapy/immunology
MH  - BCG Vaccine/*therapeutic use
MH  - Child
MH  - Cytokines/drug effects/*genetics/immunology
MH  - Gene Expression/immunology
MH  - Humans
MH  - Hypersensitivity, Immediate/immunology
MH  - Immunoblotting
MH  - Leukocytes, Mononuclear/drug effects/*immunology
MH  - Phytohemagglutinins/pharmacology
MH  - RNA, Messenger/*biosynthesis/drug effects
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tuberculin/pharmacology
EDAT- 2003/02/26 04:00
MHDA- 2003/10/28 05:00
CRDT- 2003/02/26 04:00
PHST- 2003/02/26 04:00 [pubmed]
PHST- 2003/10/28 05:00 [medline]
PHST- 2003/02/26 04:00 [entrez]
AID - S0165247802002614 [pii]
AID - 10.1016/s0165-2478(02)00261-4 [doi]
PST - ppublish
SO  - Immunol Lett. 2003 Mar 3;86(1):29-35. doi: 10.1016/s0165-2478(02)00261-4.