PMID- 12601362
OWN - NLM
STAT- MEDLINE
DCOM- 20030324
LR  - 20171116
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 37
IP  - 3
DP  - 2003 Mar
TI  - GM-CSF expands dendritic cells and their progenitors in mouse liver.
PG  - 641-52
AB  - Dendritic cells (DCs) are rare but ubiquitous antigen-presenting cells situated 
      in lymphoid and nonlymphoid organs throughout the body. The study of DCs located 
      in the liver has been restricted by their relative scarcity and the difficulty of 
      their isolation. Because granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) is a critical growth factor for DCs in vitro, we postulated that it 
      would expand hepatic DCs in vivo. We found that adenoviral-mediated GM-CSF 
      overexpression in normal mice increased the number of liver DCs 400-fold to more 
      than 100 million cells. GM-CSF-recruited DCs were CD11c(+)DEC205(-) and had high 
      expression of major histocompatibility complex (MHC) class II, CD54, and CD80 but 
      low CD40 and CD86 staining. Further maturation occurred after overnight culture. 
      In addition to CD11c(+)DEC205(-) DCs, a population of CD11c(-)DEC205(low/-) cells 
      resembling DC progenitors described previously in normal mice was expanded as 
      serum GM-CSF levels increased. GM-CSF-recruited CD11c(+)DEC205(-) DCs and 
      CD11c(-)DEC205(low/-) cells had different functional capabilities. 
      CD11c(+)DEC205(-) DCs captured far more protein antigen in vivo, produced higher 
      amounts of interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha, and induced 
      greater allogeneic and antigen-specific T-cell stimulation. A proportion of 
      CD11c(-)DEC205(low/-) cells differentiated into CD11c(+) cells and gained T-cell 
      stimulatory ability when cultured in the presence of GM-CSF. In conclusion, our 
      findings show that GM-CSF can profoundly influence recruitment and development of 
      DCs in murine liver.
FAU - Pillarisetty, Venu G
AU  - Pillarisetty VG
AD  - Hepatobiliary Service, Memorial Sloan-Kettering Cancer Center, New York, NY 
      10021, USA.
FAU - Miller, George
AU  - Miller G
FAU - Shah, Alaap B
AU  - Shah AB
FAU - DeMatteo, Ronald P
AU  - DeMatteo RP
LA  - eng
GR  - CA 094503/CA/NCI NIH HHS/United States
GR  - CA009501/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Antigens, CD)
RN  - 0 (CD11 Antigens)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Interleukin-6)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - CD11 Antigens/analysis
MH  - Cell Count
MH  - Cell Differentiation
MH  - Dendritic Cells/*cytology/immunology/metabolism
MH  - Gene Expression
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/*pharmacology
MH  - Interleukin-6/biosynthesis
MH  - *Lectins, C-Type
MH  - Liver/*cytology
MH  - Male
MH  - Membrane Glycoproteins/analysis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Minor Histocompatibility Antigens
MH  - Receptors, Cell Surface/analysis
MH  - Stem Cells/*cytology
MH  - T-Lymphocytes/immunology
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2003/02/26 04:00
MHDA- 2003/03/26 04:00
CRDT- 2003/02/26 04:00
PHST- 2003/02/26 04:00 [pubmed]
PHST- 2003/03/26 04:00 [medline]
PHST- 2003/02/26 04:00 [entrez]
AID - S0270913902141851 [pii]
AID - 10.1053/jhep.2003.50074 [doi]
PST - ppublish
SO  - Hepatology. 2003 Mar;37(3):641-52. doi: 10.1053/jhep.2003.50074.