PMID- 12603603
OWN - NLM
STAT- MEDLINE
DCOM- 20030509
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 108
IP  - 3
DP  - 2003 Mar
TI  - Macrophages exposed to Mycobacterium tuberculosis release chemokines able to 
      recruit selected leucocyte subpopulations: focus on gammadelta cells.
PG  - 365-74
AB  - Granuloma is a typical feature of tuberculosis. We evaluated the chemotaxis of 
      selected human leucocyte subsets induced by macrophages incubated with 
      Mycobacterium tuberculosis (MT)-derived products in vitro. The release of 
      monocyte chemotactic protein 1 (MCP-1) and interleukin-8 (IL-8) correlated with 
      the specific induction of strong chemotaxis towards monocytes and 
      polymorphonuclear leucocytes (PMNs). gammadelta and T helper type 1 (Th1) 
      alphabeta lymphocytes were chemoattracted, while T-resting, IL-2-activated and 
      Th2 lymphocytes were unaffected. Activation with mycobacterium-derived, 
      phosphate-containing components, modulated the chemokine receptor profile of 
      gammadelta T lymphocytes as well as their pattern of cyto-chemokine production, 
      disclosing a potential for their active participation in granuloma formation. In 
      particular, CXCR3 and IP-10, which we found to be released by MT-pulsed alveolar 
      macrophages, seem to represent the receptor-counter-receptor pair implicated in 
      the chemotaxis of gammadelta lymphocytes. Immunohistochemical analysis and in 
      situ hybridization revealed the in vivo presence of IL-8, MCP-1 and IL-10 in 
      lymph node and lung tuberculous granulomas. Our results underscore the role of MT 
      extracts in the induction of macrophage-derived chemokines responsible for the 
      orchestrated recruitment of PMNs, monocytes, and Th1 and gammadelta T cells, as 
      well as in the regulation of gammadelta function.
FAU - Ferrero, Elisabetta
AU  - Ferrero E
AD  - Laboratory of Tumour Immunology, Universita Vita e Salute, Scientific Institute H 
      San Raffaele, Via Olgettina 60, I-20132 Milan, Italy. e.ferrero@hsr.it
FAU - Biswas, Priscilla
AU  - Biswas P
FAU - Vettoretto, Katuscia
AU  - Vettoretto K
FAU - Ferrarini, Marina
AU  - Ferrarini M
FAU - Uguccioni, Mariagrazia
AU  - Uguccioni M
FAU - Piali, Luca
AU  - Piali L
FAU - Leone, Biagio Eugenio
AU  - Leone BE
FAU - Moser, Bernhard
AU  - Moser B
FAU - Rugarli, Claudio
AU  - Rugarli C
FAU - Pardi, Ruggero
AU  - Pardi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
SB  - IM
MH  - Antigens, Bacterial/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokines/*metabolism
MH  - Chemotaxis, Leukocyte/immunology
MH  - Gene Expression
MH  - Granuloma/immunology
MH  - Humans
MH  - Interleukin-8/genetics/metabolism
MH  - Lung/immunology
MH  - Lymph Nodes/immunology
MH  - Macrophages, Alveolar/*immunology
MH  - Monocytes/immunology
MH  - Mycobacterium tuberculosis/*immunology
MH  - Neutrophils/immunology
MH  - RNA, Messenger/genetics
MH  - Receptors, Antigen, T-Cell, gamma-delta/*analysis
MH  - T-Lymphocyte Subsets/*immunology
PMC - PMC1782907
EDAT- 2003/02/27 04:00
MHDA- 2003/05/13 05:00
PMCR- 2004/03/01
CRDT- 2003/02/27 04:00
PHST- 2003/02/27 04:00 [pubmed]
PHST- 2003/05/13 05:00 [medline]
PHST- 2003/02/27 04:00 [entrez]
PHST- 2004/03/01 00:00 [pmc-release]
AID - 1600 [pii]
AID - 10.1046/j.1365-2567.2003.01600.x [doi]
PST - ppublish
SO  - Immunology. 2003 Mar;108(3):365-74. doi: 10.1046/j.1365-2567.2003.01600.x.