PMID- 12604802
OWN - NLM
STAT- MEDLINE
DCOM- 20030327
LR  - 20220408
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 84
IP  - Pt 3
DP  - 2003 Mar
TI  - Genetic diversity and phylogenetic analysis of glycoprotein 5 of European-type 
      porcine reproductive and respiratory virus strains in Spain.
PG  - 529-534
LID - 10.1099/vir.0.18478-0 [doi]
AB  - The gene encoding glycoprotein 5 (ORF5) of 21 porcine reproductive and 
      respiratory syndrome virus (PRRSV) isolates from Spain and two European-type 
      vaccines currently available in that country were analysed using RT-PCR and 
      sequencing. Sequences were then compared with other European-type sequences 
      available through GenBank. Results showed percentages of similarity to Lelystad 
      virus (LV), which, in most cases, were below 90 %. In contrast, two strains were 
      very similar (>99 %) to a PRRSV variant from the Czech Republic. Evolutionary 
      trees showed three types of strains: one grouped old Spanish sequences; a second 
      grouped isolates from this study together with two Czech variant strains; and the 
      third comprised other GenBank sequences. Regarding the predicted protein 
      sequences, some isolates from this study showed a low degree of similarity to LV 
      (below 50 %) and most of the strains examined had additional N-linked 
      glycosylation sites compared to LV. These results provide evidence of the 
      existence of variant PRRSV strains in Spain with characteristics that may be 
      advantageous for immune evasion.
FAU - Mateu, Enric
AU  - Mateu E
AD  - Centre de Recerca en Sanitat Animal (CReSA), Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain.
AD  - Departament de Sanitat i Anatomia Animals, Edifici V, Universitat Autonoma de 
      Barcelona, 08193 Bellaterra, Spain.
FAU - Martin, Marga
AU  - Martin M
AD  - Centre de Recerca en Sanitat Animal (CReSA), Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain.
AD  - Departament de Sanitat i Anatomia Animals, Edifici V, Universitat Autonoma de 
      Barcelona, 08193 Bellaterra, Spain.
FAU - Vidal, Dolors
AU  - Vidal D
AD  - Departament de Sanitat i Anatomia Animals, Edifici V, Universitat Autonoma de 
      Barcelona, 08193 Bellaterra, Spain.
LA  - eng
SI  - GENBANK/AF495499
SI  - GENBANK/AF495500
SI  - GENBANK/AF495501
SI  - GENBANK/AF495502
SI  - GENBANK/AF495503
SI  - GENBANK/AF495504
SI  - GENBANK/AF495505
SI  - GENBANK/AF495506
SI  - GENBANK/AF495507
SI  - GENBANK/AF495508
SI  - GENBANK/AF495509
SI  - GENBANK/AF495510
SI  - GENBANK/AF495511
SI  - GENBANK/AF495512
SI  - GENBANK/AF495513
SI  - GENBANK/AF495514
SI  - GENBANK/AF495515
SI  - GENBANK/AF495516
SI  - GENBANK/AF495517
SI  - GENBANK/AF495518
SI  - GENBANK/AF495519
SI  - GENBANK/AF495520
SI  - GENBANK/AF495521
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Genetic Variation
MH  - Glycosylation
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/immunology/isolation & purification
MH  - RNA, Viral/*analysis
MH  - Sequence Homology, Nucleic Acid
MH  - Spain
MH  - Swine/blood/*virology
MH  - Viral Envelope Proteins/*genetics
MH  - Viral Vaccines/genetics
EDAT- 2003/02/27 04:00
MHDA- 2003/03/28 05:00
CRDT- 2003/02/27 04:00
PHST- 2003/02/27 04:00 [pubmed]
PHST- 2003/03/28 05:00 [medline]
PHST- 2003/02/27 04:00 [entrez]
AID - 10.1099/vir.0.18478-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2003 Mar;84(Pt 3):529-534. doi: 10.1099/vir.0.18478-0.