PMID- 12605123
OWN - NLM
STAT- MEDLINE
DCOM- 20030320
LR  - 20130520
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 75
IP  - 4
DP  - 2003 Feb 27
TI  - Syngeneic bone marrow transduced with a recombinant retroviral vector to express 
      endoplasmic reticulum signal-sequence-deleted major histocompatibility complex 
      class-I alloantigen can induce specific immunologic unresponsiveness in vivo.
PG  - 537-41
AB  - BACKGROUND: Long-term survival of fully allogeneic cardiac grafts can be induced 
      in mice through transduction of recipient bone marrow cells (BMCs) with a 
      recombinant retroviral vector encoding a single full-length major 
      histocompatibility complex (MHC) class I alloantigen. This study investigated 
      whether cell surface expression of the transduced MHC antigen was necessary for 
      the induction of specific unresponsiveness. METHOD The signal sequence for 
      translocation into the endoplasmic reticulum was deleted from H-2K (SDELKb). 
      Syngeneic BMCs from CBA.Ca (H2k) recipients were transduced with an MFG 
      retroviral vector encoding either wild-type Kb or the mutant SDELKb and reinfused 
      in conjunction with an anti-CD4 therapy. Four weeks later, the recipients 
      underwent transplantation with a fully allogeneic C57BL/10 cardiac graft. Graft 
      survival and the development of transplant arteriosclerosis were assessed. 
      RESULTS: Expression of both the wild-type Kb or SDELK in recipient CBA mice 
      before transplantation resulted in prolonged survival of C57BL/10 grafts. Grafts 
      from recipients pretreated with SDELKb developed 48%+/-22% intimal proliferation 
      compared with 61%+/-21% in grafts from recipients pretreated with wild-type Kb. 
      However, this difference did not reach statistical significance. CONCLUSION Cell 
      surface expression, and therefore direct recognition, of an MHC class I 
      alloantigen is not required to induce long-term survival of fully allogeneic 
      cardiac grafts after retroviral transduction of recipient BMCs.
FAU - Spriewald, Bernd M
AU  - Spriewald BM
AD  - Medical Department III, Friedrich-Alexander University, Erlangen-Nurnberg, 
      Germany.
FAU - Billing, J Stephen
AU  - Billing JS
FAU - Jenkins, Suzanne
AU  - Jenkins S
FAU - Wheeler, Paul
AU  - Wheeler P
FAU - Steger, Ulrich
AU  - Steger U
FAU - Bushell, Andrew
AU  - Bushell A
FAU - Hyde, Karen
AU  - Hyde K
FAU - Morris, Peter J
AU  - Morris PJ
FAU - Wood, Kathryn J
AU  - Wood KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antigens, Surface)
RN  - 0 (Epitopes)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Isoantigens)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/immunology
MH  - Arteriosclerosis/immunology
MH  - *Bone Marrow Transplantation
MH  - Cytosol/immunology
MH  - Endoplasmic Reticulum/*immunology
MH  - Epitopes/immunology
MH  - Genetic Vectors
MH  - Graft Survival/immunology
MH  - Heart Transplantation/*immunology
MH  - Histocompatibility Antigens Class I/*genetics/*immunology
MH  - Isoantigens/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Protein Sorting Signals
MH  - Recombinant Proteins/genetics
MH  - Retroviridae/genetics
EDAT- 2003/02/28 04:00
MHDA- 2003/03/21 04:00
CRDT- 2003/02/28 04:00
PHST- 2003/02/28 04:00 [pubmed]
PHST- 2003/03/21 04:00 [medline]
PHST- 2003/02/28 04:00 [entrez]
AID - 10.1097/01.TP.0000048379.08149.22 [doi]
PST - ppublish
SO  - Transplantation. 2003 Feb 27;75(4):537-41. doi: 
      10.1097/01.TP.0000048379.08149.22.