PMID- 12606425
OWN - NLM
STAT- MEDLINE
DCOM- 20040116
LR  - 20220227
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 68
IP  - 4
DP  - 2003 Apr
TI  - Hormonal regulation and cell-specific expression of endothelin-converting enzyme 
      1 isoforms in bovine ovarian endothelial and steroidogenic cells.
PG  - 1361-8
AB  - Endothelin-converting enzyme 1 (ECE-1) is a key enzyme in the biosynthesis of 
      endothelin 1 (ET-1), a potent regulator of ovarian function. Different ECE-1 
      isoforms are localized in distinct intracellular compartments. Thus, the spatial 
      and temporal pattern of ECE-1 expression determines the site of big ET-1 
      activation and the bioavailability of ET-1. This study was undertaken to 
      investigate the hormonal regulation and cell-specific expression of ECE-1 
      isoforms in endothelial and steroidogenic cells of bovine follicles and corpora 
      lutea (CL). Using enriched follicular and luteal cell subpopulations and in situ 
      hybridization techniques, we showed that the ECE-1 gene is expressed by both 
      endothelial and steroidogenic cells; however, the intracellular ECE-1a isoform 
      was present only in ET-1-expressing endothelial cells. Steroidogenic cells in 
      follicles or in CL, deficient in ET-1, expressed only the plasma membrane ECE-1b 
      isoform. The intensity of antisense ECE-1 labeling in the granulosa cell layer 
      increased with follicular size; insulin-like growth factor I and insulin 
      upregulated ECE-1 expression when cultured with granulosa cells, suggesting that 
      these growth factors may increase ECE-1 in growing follicles. In contrast, ET-1 
      and LH downregulated ECE-1 in steroidogenic cells. This effect could account for 
      low ECE (and ET-1) levels, which characterize the early luteal phase. These 
      findings suggest that ECE-1 is regulated during different stages of the cycle in 
      a physiologically relevant manner. The hormonal regulation and intracellular 
      localization of bovine ECE-1 isoforms revealed in this study may provide new 
      insights into ET-1 biosynthesis and mode of action in different cellular 
      microenvironments within the ovary.
FAU - Levy, Nitzan
AU  - Levy N
AD  - Department of Animal Sciences, Faculty of Agricultural, Food and Environmental 
      Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel.
FAU - Gordin, Miri
AU  - Gordin M
FAU - Smith, Michael F
AU  - Smith MF
FAU - Bolden-Tiller, Olga U
AU  - Bolden-Tiller OU
FAU - Meidan, Rina
AU  - Meidan R
LA  - eng
PT  - Journal Article
DEP - 20021030
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Endothelin-1)
RN  - 0 (Insulin)
RN  - 0 (Isoenzymes)
RN  - 0 (Steroids)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.71 (Endothelin-Converting Enzymes)
SB  - IM
MH  - Animals
MH  - Aspartic Acid Endopeptidases/*metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Endothelin-1/metabolism/pharmacology
MH  - Endothelin-Converting Enzymes
MH  - Endothelium/cytology/drug effects/metabolism
MH  - Female
MH  - Insulin/*pharmacology
MH  - Insulin-Like Growth Factor I/*pharmacology
MH  - Isoenzymes/metabolism
MH  - Luteinizing Hormone/*pharmacology
MH  - Metalloendopeptidases
MH  - Ovarian Follicle/cytology/metabolism
MH  - Ovary/cytology/drug effects/*metabolism
MH  - Steroids/*biosynthesis
MH  - Up-Regulation
EDAT- 2003/02/28 04:00
MHDA- 2004/01/17 05:00
CRDT- 2003/02/28 04:00
PHST- 2003/02/28 04:00 [pubmed]
PHST- 2004/01/17 05:00 [medline]
PHST- 2003/02/28 04:00 [entrez]
AID - biolreprod.102.009134 [pii]
AID - 10.1095/biolreprod.102.009134 [doi]
PST - ppublish
SO  - Biol Reprod. 2003 Apr;68(4):1361-8. doi: 10.1095/biolreprod.102.009134. Epub 2002 
      Oct 30.