PMID- 12608917
OWN - NLM
STAT- MEDLINE
DCOM- 20030610
LR  - 20220330
IS  - 0022-3484 (Print)
IS  - 0022-3484 (Linking)
VI  - 38
IP  - 2
DP  - 2003 Apr
TI  - Patterns of chemokines and chemokine receptors expression in different forms of 
      human periodontal disease.
PG  - 210-7
AB  - Current knowledge states that periodontal diseases are chronic inflammatory 
      reactions raised in response to periodontopathogens. Many cell types and 
      mediators, including Th1 and Th2 lymphocytes, cytokines and chemokines, appear to 
      be involved in the immunopathogenesis of periodontal diseases. Chemokines, a 
      family of chemotactic cytokines, bind to specific receptors and selectively 
      attract different cell subsets to the inflammatory site. They can also interact 
      with classical cytokines and modulate the local immune response. In order to 
      study the role of chemokines in periodontal diseases, we examined the expression 
      of chemokines, chemokine receptors and cytokines by means of reverse 
      transcription-polymerase chain reaction (RT-PCR) techniques. Characteristic 
      patterns of such factors' expression were found in gingival biopsies from 
      patients presenting with aggressive periodontitis and chronic periodontitis. The 
      expression of the chemokines macrophage inflammatory protein-1 alpha (MIP-1alpha) 
      and interferon-gamma inducible protein 10 (IP-10) and of their respective 
      receptors, CCR5 and CXCR3, were more prevalent and higher in aggressive 
      periodontitis, and associated with higher interferon-gamma (IFN-gamma) expression 
      and lower interleukin-10 (IL-10) expression. In contrast, chronic periodontitis 
      patients exhibited a more frequent and higher expression of monocyte 
      chemoattractant protein-1 (MCP-1) and its receptor CCR4, and higher expression of 
      IL-10. It is possible that chemokines, in addition to the classical cytokines, 
      are involved in the immunopathogenesis of periodontal disease, driving the 
      migration and the maintenance of several inflammatory cell types such as 
      polymorphonuclear leukocytes, dendritic cells (DCs), natural killer cells, 
      macrophages, and subsets of lymphocytes in the gingival tissues. These cells are 
      thought to participate in the inflammatory and immune reaction that takes place 
      in periodontal disease, killing pathogens, presenting antigens, and producing 
      cytokines. The selective recruitment of polarized lymphocyte subsets could result 
      in differential cytokine production at the site of response, which is supposed to 
      determine the stable or progressive nature of the lesion. Besides, the role of 
      chemokines as activators and chemoattracts of osteclasts may be involved in the 
      determination of disease severity.
FAU - Garlet, Gustavo P
AU  - Garlet GP
AD  - School of Medicine-USP, Department of Biochemistry and Immunology, Ribeirao 
      Preto, SP, Brazil.
FAU - Martins, Walter Jr
AU  - Martins W Jr
FAU - Ferreira, Beatriz R
AU  - Ferreira BR
FAU - Milanezi, Cristiane M
AU  - Milanezi CM
FAU - Silva, Joao S
AU  - Silva JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (CCR4 protein, human)
RN  - 0 (CXCR3 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Receptors, CCR4)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, CXCR3)
RN  - 0 (Receptors, Chemokine)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Chemokine CCL2/analysis/genetics
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CXCL10
MH  - Chemokines/*analysis/genetics
MH  - Chemokines, CC/immunology
MH  - Chemokines, CXC/analysis/genetics
MH  - Chronic Disease
MH  - Dendritic Cells/immunology
MH  - Female
MH  - Humans
MH  - Interferon-gamma/analysis/genetics
MH  - Interleukin-10/analysis/genetics
MH  - Killer Cells, Natural/immunology
MH  - Lymphocyte Subsets/immunology
MH  - Macrophage Inflammatory Proteins/analysis/genetics
MH  - Macrophages/immunology
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/immunology
MH  - Periodontitis/*immunology
MH  - Receptors, CCR4
MH  - Receptors, CCR5/analysis/genetics
MH  - Receptors, CXCR3
MH  - Receptors, Chemokine/*analysis/genetics
MH  - T-Lymphocytes/immunology
EDAT- 2003/03/01 04:00
MHDA- 2003/06/11 05:00
CRDT- 2003/03/01 04:00
PHST- 2003/03/01 04:00 [pubmed]
PHST- 2003/06/11 05:00 [medline]
PHST- 2003/03/01 04:00 [entrez]
AID - 2o012 [pii]
AID - 10.1034/j.1600-0765.2003.02012.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2003 Apr;38(2):210-7. doi: 10.1034/j.1600-0765.2003.02012.x.