PMID- 12608919
OWN - NLM
STAT- MEDLINE
DCOM- 20030610
LR  - 20190906
IS  - 0022-3484 (Print)
IS  - 0022-3484 (Linking)
VI  - 38
IP  - 2
DP  - 2003 Apr
TI  - Combined TUNEL and TRAP methods suggest that apoptotic bone cells are inside 
      vacuoles of alveolar bone osteoclasts in young rats.
PG  - 223-6
AB  - Although it is generally accepted that osteoclasts breakdown and resorb bone 
      matrix, the possibility that they may also be able to engulf apoptotic 
      osteoblasts/lining cells and/or osteocytes remains controversial. Apoptosis of 
      osteoblasts/ lining cells and/or osteocytes and interactions between these cells 
      and osteoclasts are extremely rapid events that are difficult to observe in vivo. 
      A suitable in vivo model for studying these events is the alveolar bone of young 
      rats because it is continuously undergoing intense resorption/remodeling. Thus, 
      sections of aldehyde fixed alveolar bone of young rats were stained by the 
      combined terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) method and the tartrate-resistant acid phosphatase (TRAP) method for the 
      simultaneous visualization of apoptotic cells and osteoclasts in the same 
      section. The combined TUNEL and TRAP reactions, in the same section, greatly 
      facilitated visualization of relationship between osteoclasts and apoptotic bone 
      cells during alveolar bone remodeling. Our results showed that several 
      TRAP-positive osteoclasts exhibited large vacuoles containing TUNEL positive 
      apoptotic structures, probably derived from osteoblasts/lining cells and/or 
      osteocytes. These results support the idea that alveolar bone osteoclasts are 
      able to internalize dying apoptotic bone cells.
FAU - Cerri, P S
AU  - Cerri PS
AD  - Department of Morphology, Sao Paulo State University (UNESP-Araraquara) SP, 
      Brazil.
FAU - Boabaid, F
AU  - Boabaid F
FAU - Katchburian, E
AU  - Katchburian E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Biomarkers)
RN  - 0 (Coloring Agents)
RN  - 0 (Isoenzymes)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/*analysis
MH  - Alveolar Process/*cytology
MH  - Animals
MH  - *Apoptosis
MH  - Biomarkers/analysis
MH  - Bone Remodeling/physiology
MH  - Bone Resorption/pathology
MH  - Coloring Agents
MH  - *In Situ Nick-End Labeling
MH  - Isoenzymes/*analysis
MH  - Osteoblasts/*cytology
MH  - Osteoclasts/*cytology
MH  - Osteocytes/*cytology
MH  - Phagocytosis/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Vacuoles/physiology/*ultrastructure
EDAT- 2003/03/01 04:00
MHDA- 2003/06/11 05:00
CRDT- 2003/03/01 04:00
PHST- 2003/03/01 04:00 [pubmed]
PHST- 2003/06/11 05:00 [medline]
PHST- 2003/03/01 04:00 [entrez]
AID - 2s006 [pii]
AID - 10.1034/j.1600-0765.2003.02006.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2003 Apr;38(2):223-6. doi: 10.1034/j.1600-0765.2003.02006.x.