PMID- 12609713
OWN - NLM
STAT- MEDLINE
DCOM- 20030508
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 191
IP  - 1
DP  - 2003 Feb 28
TI  - The effect of ursodeoxycholic acid on the survivin in thapsigargin-induced 
      apoptosis.
PG  - 83-92
AB  - Endoplasmic reticulum (ER) was recently suggested as a third subcellular 
      compartment in apoptotic execution. Survivin is a member of inhibitors of 
      apoptosis and ursodeoxycholic acid (UDCA) prevents apoptosis from various 
      apoptotic stimuli. To assess the activity of survivin and the effect of UDCA on 
      the survivin in ER stress-mediated apoptosis, we treated hepatoma cell lines with 
      thapsigargin (TG). TG-induced apoptosis was assessed by morphological changes, 
      DNA fragmentation, cleavages of poly(ADP-ribose)polymerase (PARP), and activation 
      of calpain and caspase-12. The level of survivin was decreased after TG treatment 
      in hepatoma cell lines indicating that survivin play an important role in ER 
      stress-mediated apoptosis. UDCA prevented decrease in survivin levels and 
      inhibited TG-induced apoptosis and caspase-12 activation suggesting an 
      anti-apoptotic effect of UDCA.
FAU - Sohn, Joohyun
AU  - Sohn J
AD  - Department of Medicine, Baylor College of Medicine and Veterans Affairs Medical 
      Center (151B), 2002 Holcombe Boulevard, Houston, TX 77030, USA.
FAU - Khaoustov, Vladimir I
AU  - Khaoustov VI
FAU - Xie, Qing
AU  - Xie Q
FAU - Chung, Charles C
AU  - Chung CC
FAU - Krishnan, Bhuvaneswari
AU  - Krishnan B
FAU - Yoffe, Boris
AU  - Yoffe B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Survivin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 724L30Y2QR (Ursodeoxycholic Acid)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 3.4.22.- (CASP12 protein, human)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (Caspase 12)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis/*drug effects/physiology
MH  - Calpain/metabolism
MH  - Carcinoma, Hepatocellular/pathology
MH  - Caspase 12
MH  - Caspases/metabolism
MH  - DNA Fragmentation/drug effects
MH  - Endoplasmic Reticulum/physiology
MH  - Enzyme Activation/drug effects
MH  - Genes, bcl-2
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins
MH  - Liver Neoplasms/pathology
MH  - Microtubule-Associated Proteins/*biosynthesis/genetics/physiology
MH  - Neoplasm Proteins/*biosynthesis/genetics/physiology
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/deficiency/physiology
MH  - Stress, Physiological/genetics/metabolism
MH  - Survivin
MH  - Thapsigargin/*pharmacology
MH  - Tumor Cells, Cultured/drug effects/metabolism/pathology
MH  - Ursodeoxycholic Acid/pharmacology
EDAT- 2003/03/01 04:00
MHDA- 2003/05/09 05:00
CRDT- 2003/03/01 04:00
PHST- 2003/03/01 04:00 [pubmed]
PHST- 2003/05/09 05:00 [medline]
PHST- 2003/03/01 04:00 [entrez]
AID - S0304383502006249 [pii]
AID - 10.1016/s0304-3835(02)00624-9 [doi]
PST - ppublish
SO  - Cancer Lett. 2003 Feb 28;191(1):83-92. doi: 10.1016/s0304-3835(02)00624-9.