PMID- 12611640
OWN - NLM
STAT- MEDLINE
DCOM- 20030513
LR  - 20200225
IS  - 1582-1838 (Print)
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Linking)
VI  - 6
IP  - 4
DP  - 2002 Oct-Dec
TI  - Dantrolene protects neurons against kainic acid induced apoptosis in vitro and in 
      vivo.
PG  - 555-69
AB  - Apoptotic cell death induced by kainic acid (KA) in cultures of rat cerebellar 
      granule cells (CGC) and in different brain regions of Wistar rat pups on 
      postnatal day 21 (P21) was studied. In vitro, KA (100-500 microM) induced a 
      concentration-dependent loss of cell viability in MTT assay and cell death had 
      apoptotic morphology as studied by chromatin staining with propidium iodide (PI). 
      In vivo, twenty-four hours after induction of status epilepticus (SE) by an 
      intraperitoneal KA injection (5 mg/kg) we quantified apoptotic cells in 
      hippocampus (CA1 and CA3), parietal cortex and cerebellum using PI staining and 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      technique. We report that dantrolene, a specific ryanodine receptor antagonist, 
      was able to significantly reduce the apoptotic cell death in CGC cultures and in 
      hyppocampal CA1 and parietal cortex regions. Our finding can be valuable for 
      neuroprotective therapy strategies in patients with repeated generalized seizures 
      or status epilepticus.
FAU - Popescu, B O
AU  - Popescu BO
AD  - Department of Neurology, University Hospital, "Carol Davila" University of 
      Medicine and Pharmacy, Bucharest, Romania. Bogdan.Popescu@univermed-cdgm.ro
FAU - Oprica, M
AU  - Oprica M
FAU - Sajin, Maria
AU  - Sajin M
FAU - Stanciu, Cristina L
AU  - Stanciu CL
FAU - Bajenaru, O
AU  - Bajenaru O
FAU - Predescu, Andreea
AU  - Predescu A
FAU - Vidulescu, Cristina
AU  - Vidulescu C
FAU - Popescu, L M
AU  - Popescu LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - F64QU97QCR (Dantrolene)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Brain/drug effects
MH  - Cells, Cultured
MH  - DNA Damage/drug effects
MH  - Dantrolene/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Kainic Acid/*pharmacology
MH  - Neurons/*drug effects/*pathology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC6741407
EDAT- 2003/03/04 04:00
MHDA- 2003/05/14 05:00
PMCR- 2002/10/01
CRDT- 2003/03/04 04:00
PHST- 2003/03/04 04:00 [pubmed]
PHST- 2003/05/14 05:00 [medline]
PHST- 2003/03/04 04:00 [entrez]
PHST- 2002/10/01 00:00 [pmc-release]
AID - 006.004.07 [pii]
AID - JCMM555 [pii]
AID - 10.1111/j.1582-4934.2002.tb00454.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2002 Oct-Dec;6(4):555-69. doi: 
      10.1111/j.1582-4934.2002.tb00454.x.