PMID- 12615141
OWN - NLM
STAT- MEDLINE
DCOM- 20030626
LR  - 20190717
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 48
IP  - 1
DP  - 2003 Jan
TI  - Expression of tumour necrosis factor-alpha in the rat dental follicle.
PG  - 47-54
AB  - Tooth eruption requires the presence of the dental follicle, a loose connective 
      tissue sac that surrounds each unerupted tooth. The follicle appears to regulate 
      many of the cellular and molecular events of eruption, including the formation of 
      osteoclasts needed to resorb alveolar bone to form an eruption pathway. To that 
      end, the expression of the tumour necrosis factor-alpha (TNF-alpha) gene was 
      examined in the dental follicle as a possible regulator of osteoclastogenesis. 
      TNF-alpha was expressed slightly in the dental follicle of the first mandibular 
      molar of the rat beginning at day 3 postnatally, but maximal expression was seen 
      at day 9, a time that correlates with a slight burst of osteoclast formation seen 
      at day 10 postnatally. In vitro, TNF-alpha was not expressed constitutively in 
      the follicle cells but incubating them with interleukin 1alpha resulted in a 
      strong expression of TNF-alpha after only 0.5h. TNF-alpha itself enhanced 
      monocyte chemotactic protein 1 (MCP-1) and vascular endothelial growth factor 
      (VEGF) gene expression. It also slightly decreased the expression of 
      osteoprotegerin after 3-h incubation but this returned to the control level at 
      6h. MCP-1 and VEGF could aid in recruiting mononuclear cells (osteoclast 
      precursors) to the dental follicle. In addition to the potential role of 
      TNF-alpha in tooth eruption, this study suggests that the periodontal ligament 
      derived from the dental follicle might have the capacity to synthesize TNF-alpha, 
      and thereby contribute to the destructive events of periodontitis.
FAU - Wise, Gary E
AU  - Wise GE
AD  - Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State 
      University, Baton Rouge, LA 70803, USA. gwise@vetmed.lsu.edu 
      <gwise@vetmed.lsu.edu>
FAU - Yao, Shaomian
AU  - Yao S
LA  - eng
GR  - R01 DE08911/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Chemokine CCL2)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (Lymphokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Dental Sac/cytology/drug effects/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Endothelial Growth Factors/metabolism
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Interleukin-1/pharmacology
MH  - Lymphokines/metabolism
MH  - Osteoclasts/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tooth Eruption/genetics/physiology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/*genetics/pharmacology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2003/03/05 04:00
MHDA- 2003/06/27 05:00
CRDT- 2003/03/05 04:00
PHST- 2003/03/05 04:00 [pubmed]
PHST- 2003/06/27 05:00 [medline]
PHST- 2003/03/05 04:00 [entrez]
AID - S000399690200153X [pii]
AID - 10.1016/s0003-9969(02)00153-x [doi]
PST - ppublish
SO  - Arch Oral Biol. 2003 Jan;48(1):47-54. doi: 10.1016/s0003-9969(02)00153-x.