PMID- 12617998
OWN - NLM
STAT- MEDLINE
DCOM- 20030429
LR  - 20190826
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 39
IP  - 13
DP  - 2003 Mar
TI  - Isolation and characterization of a novel CXC chemokine in common carp (Cyprinus 
      carpio L.).
PG  - 829-34
AB  - A novel CXC chemokine was identified for the first time in fish from common carp 
      (Cyprinus carpio L.). The gene was obtained from the head kidney (HK) stimulated 
      with LPS and Con A. The cDNA consists of 619 bp with a 37 bp 5' UTR and a 287 bp 
      3' UTR. An open reading frame of 368 bp encodes a 97 amino acid peptide, with a 
      putative signal peptide of 20 aa. The gene has four cysteines residues, which are 
      conserved, with first two cysteines separated with phenylalanine. By homology and 
      phylogenetic analysis, the chemokine was found to be closer to human IP-10. 
      Identities were significantly low to the CXC chemokines cloned from lamprey 
      (Lampetra fluviatilis), flounder (Paralichthys olivaceus), rainbow trout 
      (Onchorhynchus mykiss) and zebrafish (Danio rerio). The carp CXC chemokine 
      contains three exons interrupted by two introns. The gene was transcribed from an 
      early time point by stimulation with LPS and Con A. Organs in resting phase as 
      well as stimulated expressed the gene.
CI  - Copyright 2002 Elsevier Science Ltd.
FAU - Savan, Ram
AU  - Savan R
AD  - United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto 
      1-21-24, Kagoshima 890-0065, Japan.
FAU - Kono, Tomoya
AU  - Kono T
FAU - Aman, Azumi
AU  - Aman A
FAU - Sakai, Masahiro
AU  - Sakai M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Chemokines, CXC)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carps/genetics/*immunology
MH  - Chemokines, CXC/genetics/*isolation & purification
MH  - DNA, Complementary/genetics
MH  - Exons
MH  - Introns
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Homology, Amino Acid
EDAT- 2003/03/06 04:00
MHDA- 2003/04/30 05:00
CRDT- 2003/03/06 04:00
PHST- 2003/03/06 04:00 [pubmed]
PHST- 2003/04/30 05:00 [medline]
PHST- 2003/03/06 04:00 [entrez]
AID - S0161589002002572 [pii]
AID - 10.1016/s0161-5890(02)00257-2 [doi]
PST - ppublish
SO  - Mol Immunol. 2003 Mar;39(13):829-34. doi: 10.1016/s0161-5890(02)00257-2.