PMID- 12618212
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20190922
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 15
IP  - 4
DP  - 2003 Apr
TI  - Formylpeptides trigger selective molecular pathways that are required in the 
      physiological functions of human neutrophils.
PG  - 377-83
AB  - For-Met-Delta(z)Leu-Phe-OMe ([Delta(z)Leu(2)]) is a conformationally restricted 
      for-Met-Leu-Phe-OMe (fMLP-OMe) analogue able to discriminate between different 
      responses of human neutrophils. In contrast, [Delta(z)Leu(2)] significantly 
      activates the transduction pathways-involving Ca(2+), inositol phosphate, and 
      cyclic AMP (cAMP) enhancement, as is the case with the full agonist fMLP-OMe. 
      Here, we have studied the specific involvement of protein kinase C (PKC) isoforms 
      and mitogen activated protein kinases (MAPKs) in the presence or absence of 
      extracellular Ca(2+), being the cation clearly involved in the activation of 
      neutrophils by fMLP. A strong correlation has been found between PKC isoforms, 
      MAPKs and the selective physiological functions by [Delta(z)Leu(2)]-activated 
      neutrophils. In a calcium-free condition, our data suggest that the failure of 
      PKC beta1 translocation and of p38 MAPK phosphorylation by the analogue refers to 
      its inability to induce chemotaxis, and that the failure by both fMLP-OMe and 
      [Delta(z)Leu(2)] to evoke extracellular response kinase 1 and 2 (ERK1/2) 
      phosphorylation would suggest a reduction in superoxide anion production.
FAU - Selvatici, Rita
AU  - Selvatici R
AD  - Dipartimento di Medicina Sperimentale e Diagnostica, Sezione Genetica Medica, Via 
      L Borsari 46, Universita degli Studi di Ferrara, 44100 Ferrara, Italy.
FAU - Falzarano, Sofia
AU  - Falzarano S
FAU - Traniello, Serena
AU  - Traniello S
FAU - Pagani Zecchini, Giampiero
AU  - Pagani Zecchini G
FAU - Spisani, Susanna
AU  - Spisani S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 11062-77-4 (Superoxides)
RN  - 137320-56-0 (formyl-methionyl-delta(Z)-dehydroleucyl-phenylalanine methyl ester)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 65929-03-5 (formylmethionyl-leucyl-phenylalanine methyl ester)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/pharmacology
MH  - Calcium Signaling/*drug effects
MH  - Cells, Cultured
MH  - *Chemotaxis, Leukocyte/drug effects
MH  - Enzyme Activation
MH  - Humans
MH  - In Vitro Techniques
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/*analogs & derivatives/*pharmacology
MH  - Neutrophils/*drug effects/enzymology/physiology
MH  - Phosphorylation
MH  - Protein Kinase C/metabolism
MH  - Superoxides
EDAT- 2003/03/06 04:00
MHDA- 2004/01/06 05:00
CRDT- 2003/03/06 04:00
PHST- 2003/03/06 04:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/03/06 04:00 [entrez]
AID - S0898656802001237 [pii]
AID - 10.1016/s0898-6568(02)00123-7 [doi]
PST - ppublish
SO  - Cell Signal. 2003 Apr;15(4):377-83. doi: 10.1016/s0898-6568(02)00123-7.