PMID- 12619096
OWN - NLM
STAT- MEDLINE
DCOM- 20030924
LR  - 20191210
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 28
IP  - 3
DP  - 2003 Mar
TI  - Immunocytochemical evaluation of HER-2/neu on fine-needle aspirates from primary 
      breast carcinomas.
PG  - 142-6
AB  - Detection of HER-2/neu alterations is increasingly used in breast cancer patients 
      for therapeutic purposes. This study examines the reliability of HER-2/neu 
      immunocytochemical assessment on 66 cytospin smears obtained by fine-needle 
      aspiration biopsy from breast cancer patients. Results were compared with those 
      obtained by both fluorescence in situ hybridization (FISH) on fine-needle 
      aspirate (FNA) and immunohistochemistry (IHC) on matched histologic section. 
      Concordance between immunocytochemistry (ICC) and FISH was 78% and between ICC 
      and IHC was 84%. Discordance mainly concerned seven unamplified cases that 
      resulted positive by ICC and four cases scored negative by IHC but positive by 
      ICC. Simultaneous assessment of HER-2/neu by ICC, IHC, and FISH was available in 
      24 cases; the concordance was 75%. In this study, the false positivity of 
      immunocytochemical technique represents the major criticism. In our experience, 
      FISH remains the most objective and powerful technique for HER-2/neu assessment 
      on breast cancer FNAs.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Nizzoli, Rita
AU  - Nizzoli R
AD  - Divisione di Oncologia Medica, Ospedale di Parma, Parma, Italy. rnizzoli@ao.pr.it
FAU - Bozzetti, Cecilia
AU  - Bozzetti C
FAU - Crafa, Pellegrino
AU  - Crafa P
FAU - Naldi, Nadia
AU  - Naldi N
FAU - Guazzi, Annamaria
AU  - Guazzi A
FAU - Di Blasio, Beatrice
AU  - Di Blasio B
FAU - Camisa, Roberta
AU  - Camisa R
FAU - Cascinu, Stefano
AU  - Cascinu S
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adenocarcinoma/*genetics/metabolism/pathology
MH  - Biopsy, Needle/*methods
MH  - Breast Neoplasms/*genetics/metabolism/pathology
MH  - False Positive Reactions
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Messenger/metabolism
MH  - Receptor, ErbB-2
MH  - Reproducibility of Results
EDAT- 2003/03/06 04:00
MHDA- 2003/09/25 05:00
CRDT- 2003/03/06 04:00
PHST- 2003/03/06 04:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/03/06 04:00 [entrez]
AID - 10.1002/dc.10257 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2003 Mar;28(3):142-6. doi: 10.1002/dc.10257.