PMID- 12620497
OWN - NLM
STAT- MEDLINE
DCOM- 20030408
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 79 Suppl 1
DP  - 2003 Mar
TI  - Interleukin-13 and tumor necrosis factor-beta differentially regulate the 
      production of cytokines by cultured human endometrial stromal cells.
PG  - 821-7
AB  - OBJECTIVE: To evaluate the effects of interleukin (IL)-13, a T-helper (Th)2 
      cytokine, and tumor necrosis factor (TNF)-beta, a Th1 cytokine, on the production 
      of IL-6 family cytokines and chemokines by endometrial stromal cells (ESC). 
      DESIGN: The effects of IL-13 and TNF-beta, on the production of IL-6, IL-11, 
      leukemia inhibitory factor (LIF), IL-8, growth-regulated oncogene alpha 
      (GROalpha), monocyte chemoattractant protein-1 (MCP-1), regulated on activation, 
      T-cell expressed and secreted (RANTES), and eotaxin were investigated. SETTING: 
      Research laboratory at a medical university. PATIENT(S): Thirteen endometrial 
      specimens in the late proliferative phase were used. INTERVENTION(S): The ESC 
      were incubated for 24 hours with recombinant human IL-13 and recombinant human 
      TNF-beta. MAIN OUTCOME MEASURE(S): The concentration of IL-6, IL-11, LIF, IL-8, 
      GROalpha, MCP-1, RANTES, and eotaxin in the culture media was measured using 
      ELISA. RESULT(S): The increase in levels of IL-6, IL-8, MCP-1, and eotaxin in the 
      culture media of ESC paralleled the addition of increasing amounts of IL-13 and 
      TNF-beta, whereas the levels of IL-11 and LIF were decreased with increasing 
      amounts of IL-13, but were increased with increasing amounts of TNF-beta. Tumor 
      necrosis factor-beta enhanced the production of GROalpha and RANTES in 
      dose-dependent manner; however, IL-13 did not affect the expression of GROalpha 
      or RANTES. CONCLUSION(S): These results suggest that IL-13 and TNF-beta secreted 
      in the cyclic endometrial tissue and in the decidua may differentially regulate 
      the production of IL-6 family cytokines and chemokines by ESC. The controlled 
      expression of these cytokines in the endometrium may contribute to the modulation 
      of the immune reaction during the menstrual cycle and in early pregnancy by the 
      regulation of leukocyte trafficking and functions.
FAU - Nasu, Kaei
AU  - Nasu K
AD  - Department of Obstetrics and Gynecology, Oita Medical University, Oita, Japan. 
      nasu@oita-med.ac.jp
FAU - Fukuda, Junichiro
AU  - Fukuda J
FAU - Sun, Bing
AU  - Sun B
FAU - Nishida, Masakazu
AU  - Nishida M
FAU - Miyakawa, Isao
AU  - Miyakawa I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-13)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adult
MH  - Cytokines/*biosynthesis
MH  - Endometrium/cytology/drug effects/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Interleukin-13/*pharmacology
MH  - Lymphotoxin-alpha/*pharmacology
MH  - Middle Aged
MH  - Recombinant Proteins/pharmacology
MH  - Stromal Cells/drug effects/metabolism
EDAT- 2003/03/07 04:00
MHDA- 2003/04/09 05:00
CRDT- 2003/03/07 04:00
PHST- 2003/03/07 04:00 [pubmed]
PHST- 2003/04/09 05:00 [medline]
PHST- 2003/03/07 04:00 [entrez]
AID - S0015028202048318 [pii]
AID - 10.1016/s0015-0282(02)04831-8 [doi]
PST - ppublish
SO  - Fertil Steril. 2003 Mar;79 Suppl 1:821-7. doi: 10.1016/s0015-0282(02)04831-8.