PMID- 12621190
OWN - NLM
STAT- MEDLINE
DCOM- 20030424
LR  - 20220310
IS  - 0032-1052 (Print)
IS  - 0032-1052 (Linking)
VI  - 111
IP  - 3
DP  - 2003 Mar
TI  - Increasing the volume of vascularized tissue formation in engineered constructs: 
      an experimental study in rats.
PG  - 1186-92; discussion 1193-4
AB  - The authors have previously described a model of in vivo tissue generation based 
      on an implanted, microsurgically created vessel loop in a plastic chamber 
      (volume, 0.45 ml) containing a poly(DL-lactic-co-glycolic acid) (PLGA) scaffold. 
      Tissue grew spontaneously in association with an intense angiogenic sprouting 
      from the loop and almost filled the chamber, resulting in a mean amount of tissue 
      in chambers of 0.23 g with no added matrix scaffold and 0.33 g of tissue in 
      PLGA-filled chambers after 4 weeks of incubation. The aim of the present study 
      was to investigate whether a greater volume of tissue could be generated when the 
      same-size vessel loop was inserted into a larger (1.9 ml) chamber. In four groups 
      of five rats, an arteriovenous shunt sandwiched between two disks of PLGA, used 
      as a scaffold for structural support, was placed inside a large polycarbonate 
      growth chamber. Tissue and PLGA weight and volume, as well as histological 
      characteristics of the newly formed tissue, were assessed at 2, 4, 6, and 8 
      weeks. Tissue weight and volume showed a strong linear correlation. Tissue weight 
      increased progressively from 0.13 +/- 0.04 g at 2 weeks to 0.57 +/- 0.06 g at 6 
      weeks (p < 0.0005). PLGA weight decreased progressively from 0.89 +/- 0.07 g at 2 
      weeks to 0.20 +/- 0.09 g at 8 weeks (p < 0.0005). Histological examination of the 
      specimens confirmed increased tissue growth and maturation over time. It is 
      concluded that larger quantities of tissue can be grown over a longer period of 
      time by using larger-size growth chambers.
FAU - Hofer, Stefan O P
AU  - Hofer SO
AD  - Bernard O'Brien Institute of Microsurgery, University of Melbourne, St. Vincent's 
      Hospital, 42 Fitzroy Street, Fitzroy, Victoria 3065, Australia. 
      bobimsvh@svhm.org.au
FAU - Knight, Katherine M
AU  - Knight KM
FAU - Cooper-White, Justin J
AU  - Cooper-White JJ
FAU - O'Connor, Andrea J
AU  - O'Connor AJ
FAU - Perera, Jilska M
AU  - Perera JM
FAU - Romeo-Meeuw, Rosalind
AU  - Romeo-Meeuw R
FAU - Penington, Anthony J
AU  - Penington AJ
FAU - Knight, Kenneth R
AU  - Knight KR
FAU - Morrison, Wayne A
AU  - Morrison WA
FAU - Messina, Aurora
AU  - Messina A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plast Reconstr Surg
JT  - Plastic and reconstructive surgery
JID - 1306050
SB  - IM
MH  - Animals
MH  - Diffusion Chambers, Culture
MH  - Male
MH  - *Neovascularization, Physiologic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tissue Engineering/methods
EDAT- 2003/03/07 04:00
MHDA- 2003/04/25 05:00
CRDT- 2003/03/07 04:00
PHST- 2003/03/07 04:00 [pubmed]
PHST- 2003/04/25 05:00 [medline]
PHST- 2003/03/07 04:00 [entrez]
AID - 10.1097/01.PRS.0000046034.02158.EB [doi]
PST - ppublish
SO  - Plast Reconstr Surg. 2003 Mar;111(3):1186-92; discussion 1193-4. doi: 
      10.1097/01.PRS.0000046034.02158.EB.