PMID- 12621489
OWN - NLM
STAT- MEDLINE
DCOM- 20030923
LR  - 20131121
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 31
IP  - 2
DP  - 2003 Jan
TI  - Etoposide (VP-16) plus G-CSF mobilizes different dendritic cell subsets than does 
      G-CSF alone.
PG  - 95-8
AB  - Dendritic cells (DCs) are antigen-presenting cells that are critical to the 
      generation of immunologic tumor responses. Myeloid DCs (DC1) express myeloid 
      antigen CD11c; lymphoid DCs (DC2) express CD123(+) and are CD11c(-). Analysis of 
      DC subsets from peripheral blood progenitor cells (PBPC) collected from normal 
      donors mobilized with G-CSF shows a predominance of DC2 cells. Whether PBPCs 
      mobilization by chemotherapy yields different subsets of DCs has not been 
      studied. We analyzed DC subsets in apheresis products from 44 patients undergoing 
      autologous stem cell transplantation from 6/00 to 5/01. Patients received either 
      G-CSF alone (10 microg/kg per day, n=11) or etoposide (2 g/m(2)) plus G-CSF 
      (n=33) for progenitor cell mobilization. The patients were apheresed for 2-10 
      days (median 3) to reach a minimum of 2.0 x 10(6) CD34(+) cells/kg. Patients 
      receiving G-CSF alone mobilized significantly more total DC2s than did those 
      receiving etoposide plus G-CSF (median 6.2 x 10(6)/kg vs 2.9 x 10(6)/kg, 
      P=0.001). The DC2/DC1 ratio was also significantly different in the two groups, 
      with the G-CSF group having a higher ratio (median 1.2 vs 0.4, P<0.001). We 
      conclude that the combination of chemotherapy plus G-CSF yields different 
      mobilized dendritic cell subsets than does G-CSF alone.
FAU - Bolwell, B
AU  - Bolwell B
AD  - Department of Hematology and Medical Oncology, Cleveland, OH, USA.
FAU - Sobecks, R
AU  - Sobecks R
FAU - Pohlman, B
AU  - Pohlman B
FAU - Andresen, S
AU  - Andresen S
FAU - Theil, K
AU  - Theil K
FAU - Serafino, S
AU  - Serafino S
FAU - Rybicki, L
AU  - Rybicki L
FAU - Kalaycio, M
AU  - Kalaycio M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Recombinant Proteins)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 6PLQ3CP4P3 (Etoposide)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Count
MH  - Dendritic Cells/classification/drug effects/*immunology
MH  - Etoposide/*therapeutic use
MH  - Female
MH  - Flow Cytometry/methods
MH  - Granulocyte Colony-Stimulating Factor/*therapeutic use
MH  - Hematopoietic Stem Cell Mobilization/*methods
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Recombinant Proteins
MH  - *Stem Cell Transplantation
MH  - Transplantation, Autologous
MH  - Treatment Outcome
EDAT- 2003/03/07 04:00
MHDA- 2003/09/25 05:00
CRDT- 2003/03/07 04:00
PHST- 2003/03/07 04:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/03/07 04:00 [entrez]
AID - 1703791 [pii]
AID - 10.1038/sj.bmt.1703791 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 2003 Jan;31(2):95-8. doi: 10.1038/sj.bmt.1703791.