PMID- 12623845
OWN - NLM
STAT- MEDLINE
DCOM- 20030819
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 102
IP  - 1
DP  - 2003 Jul 1
TI  - Rapid recruitment of inflammatory monocytes is independent of neutrophil 
      migration.
PG  - 328-35
AB  - Early neutrophil entry into an inflammatory site is thought to mediate a 
      chemokine switch, inducing subsequent monocyte recruitment through the regulation 
      of monocyte chemoattractant protein-1 (MCP-1) release. As the murine monocyte is 
      poorly characterized and difficult to identify, there has been little examination 
      of either its early recruitment in inflammatory models or of the factors that 
      influence its early migration. The phenotyping of rapidly recruited inflammatory 
      leukocytes with 7/4 and Gr-1 monoclonal antibodies (mAbs) identifies 2 distinct 
      populations, which we characterize as murine monocytes and neutrophils. Monocytes 
      migrate in the first 2 hours of inflammation making use of alpha4beta1 but not of 
      Mac-1 or lymphocyte function-associated antigen-1 (LFA-1) integrins. Early 
      migration is dependent on MCP-1, but neither MCP-1 release nor monocyte 
      recruitment is affected by the reduced neutrophil migration seen in LFA-1-/- 
      mice. Endogenous peritoneal macrophages and mesothelial cells lining the 
      peritoneum contain MCP-1, which is released following thioglycollate stimulation. 
      The murine monocyte therefore responds rapidly to chemokines produced in situ by 
      tissue cells at the site of inflammation with no requirement for prior influx of 
      neutrophils.
FAU - Henderson, Robert B
AU  - Henderson RB
AD  - Leukocyte Adhesion Laboratory, Cancer Research United Kingdom London Research 
      Institute, Lincoln's Inn Fields Laboratories, London WC2A 3PX, UK.
FAU - Hobbs, Josie A R
AU  - Hobbs JA
FAU - Mathies, Meg
AU  - Mathies M
FAU - Hogg, Nancy
AU  - Hogg N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030306
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 143198-26-9 (Integrin alpha4)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Chemokine CCL2/analysis/biosynthesis
MH  - *Chemotaxis, Leukocyte
MH  - Inflammation/immunology/pathology
MH  - Integrin alpha4/physiology
MH  - Lymphocyte Function-Associated Antigen-1/physiology
MH  - Mice
MH  - Mice, Knockout
MH  - Monocytes/*cytology
MH  - *Neutrophil Infiltration
MH  - Peritonitis/immunology/pathology
EDAT- 2003/03/08 04:00
MHDA- 2003/08/20 05:00
CRDT- 2003/03/08 04:00
PHST- 2003/03/08 04:00 [pubmed]
PHST- 2003/08/20 05:00 [medline]
PHST- 2003/03/08 04:00 [entrez]
AID - S0006-4971(20)54013-5 [pii]
AID - 10.1182/blood-2002-10-3228 [doi]
PST - ppublish
SO  - Blood. 2003 Jul 1;102(1):328-35. doi: 10.1182/blood-2002-10-3228. Epub 2003 Mar 
      6.