PMID- 12624091
OWN - NLM
STAT- MEDLINE
DCOM- 20030625
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 278
IP  - 20
DP  - 2003 May 16
TI  - Molecular characterization of an interleukin-4-inducing factor from Schistosoma 
      mansoni eggs.
PG  - 18384-92
AB  - The eggs of the parasitic trematode Schistosoma mansoni are powerful inducers of 
      a T helper type 2 (Th2) immune response and immunoglobulin E (IgE) production. S. 
      mansoni egg extract (SmEA) stimulates human basophils to rapidly release large 
      amounts of interleukin (IL)-4, the key promoter of a Th2 response. Here we show 
      purification and sequence of the IL-4-inducing principle of S. mansoni eggs 
      (IPSE). Stimulation studies with human basophils using SmEA fractions and natural 
      and recombinant IPSE as well as neutralization and immunodepletion studies using 
      antibodies to recombinant IPSE demonstrate that IPSE is the bioactive principle 
      in SmEA leading to activation of basophils and to expression of IL-4 and IL-13. 
      Regarding the mechanism of action, blot analysis showed that IPSE is an 
      IgE-binding factor, suggesting that it becomes effective via cross-linking 
      receptor-bound IgE on basophils. Immunohistology revealed that IPSE is enriched 
      in and secreted from the subshell area of the schistosome egg. We conclude from 
      these data that IPSE may be an important parasite-derived component for skewing 
      the immune response toward Th2.
FAU - Schramm, Gabriele
AU  - Schramm G
AD  - Forschungszentrum Borstel, D-23845 Borstel, Germany.
FAU - Falcone, Franco H
AU  - Falcone FH
FAU - Gronow, Achim
AU  - Gronow A
FAU - Haisch, Karin
AU  - Haisch K
FAU - Mamat, Uwe
AU  - Mamat U
FAU - Doenhoff, Michael J
AU  - Doenhoff MJ
FAU - Oliveira, Guilherme
AU  - Oliveira G
FAU - Galle, Jurgen
AU  - Galle J
FAU - Dahinden, Clemens A
AU  - Dahinden CA
FAU - Haas, Helmut
AU  - Haas H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030306
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Egg Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (IPSE protein, Schistosoma mansoni)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-3)
RN  - 0 (Lectins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Base Sequence
MH  - Basophils/metabolism/parasitology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chromatography, Ion Exchange
MH  - DNA, Complementary/metabolism
MH  - Databases as Topic
MH  - Dose-Response Relationship, Drug
MH  - Egg Proteins/*chemistry/*genetics/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Gene Library
MH  - *Helminth Proteins
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-13/metabolism
MH  - Interleukin-3/metabolism
MH  - Interleukin-4/*metabolism
MH  - Lectins/metabolism
MH  - Molecular Sequence Data
MH  - Protein Structure, Tertiary
MH  - Protozoan Proteins/*chemistry/*genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Schistosoma mansoni/embryology/*metabolism
MH  - Th2 Cells/metabolism
EDAT- 2003/03/08 04:00
MHDA- 2003/06/26 05:00
CRDT- 2003/03/08 04:00
PHST- 2003/03/08 04:00 [pubmed]
PHST- 2003/06/26 05:00 [medline]
PHST- 2003/03/08 04:00 [entrez]
AID - S0021-9258(19)54941-9 [pii]
AID - 10.1074/jbc.M300497200 [doi]
PST - ppublish
SO  - J Biol Chem. 2003 May 16;278(20):18384-92. doi: 10.1074/jbc.M300497200. Epub 2003 
      Mar 6.