PMID- 12624208
OWN - NLM
STAT- MEDLINE
DCOM- 20030502
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 149
IP  - Pt 2
DP  - 2003 Feb
TI  - Kinetic and phylogenetic characterization of an anaerobic dechlorinating 
      microbial community.
PG  - 459-469
LID - 10.1099/mic.0.26018-0 [doi]
AB  - The reductive dechlorination (RD) of tetrachloroethene (PCE) to vinyl chloride 
      (VC) and, to a lesser extent, to ethene (ETH) by an anaerobic microbial community 
      has been investigated by studying the processes and kinetics of the main 
      physiological components of the consortium. Molecular hydrogen, produced by 
      methanol-utilizing acetogens, was the electron donor for the PCE RD to VC and ETH 
      without forming any appreciable amount of other chlorinated intermediates and in 
      the near absence of methanogenic activity. The microbial community structure of 
      the consortium was investigated by preparing a 16S rDNA clone library and by 
      fluorescence in situ hybridization (FISH). The PCR primers used in the clone 
      library allowed the harvest of 16S rDNA from both bacterial and archaeal members 
      in the community. A total of 616 clones were screened by RFLP analysis of the 
      clone inserts followed by the sequencing of RFLP group representatives and 
      phylogenetic analysis. The clone library contained sequences mostly from hitherto 
      undescribed bacteria. No sequences similar to those of the known RD bacteria like 
      'Dehalococcoides ethenogenes' or Dehalobacter restrictus were found in the clone 
      library, and none of these bacteria was present in the RD consortium according to 
      FISH. Almost all clones fell into six previously described phyla of the bacterial 
      domain, with the majority (56.6 %) being deep-branching members of the 
      Spirochaetes phylum. Other clones were in the Firmicutes phylum (18.5 %), the 
      Chloroflexi phylum (16.4 %), the Bacteroidetes phylum (6.3 %), the Synergistes 
      genus (1.1 %) and a lineage that could not be affiliated with existing phyla (1.1 
      %). No archaeal clones were found in the clone library. Owing to the phylogenetic 
      novelty of the microbial community with regard to previously cultured 
      micro-organisms, no specific microbial component(s) could be hypothetically 
      affiliated with the RD phenotype. The predominance of Spirochaetes in the 
      microbial consortium, the main group revealed by clone library analysis, was 
      confirmed by FISH using a purposely developed probe.
FAU - Rossetti, Simona
AU  - Rossetti S
AD  - Water Research Institute, Via Reno 1, 00198, Rome, Italy.
FAU - Blackall, Linda L
AU  - Blackall LL
AD  - Department of Microbiology and Parasitology, The University of Queensland, 
      Brisbane, 4072, Australia.
FAU - Majone, Mauro
AU  - Majone M
AD  - Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, 
      00185 Rome, Italy.
FAU - Hugenholtz, Philip
AU  - Hugenholtz P
AD  - Department of Microbiology and Parasitology, The University of Queensland, 
      Brisbane, 4072, Australia.
FAU - Plumb, Jason J
AU  - Plumb JJ
AD  - Department of Microbiology and Parasitology, The University of Queensland, 
      Brisbane, 4072, Australia.
FAU - Tandoi, Valter
AU  - Tandoi V
AD  - Water Research Institute, Via Reno 1, 00198, Rome, Italy.
LA  - eng
SI  - GENBANK/AF408637
SI  - GENBANK/AF408638
SI  - GENBANK/AF408639
SI  - GENBANK/AF408640
SI  - GENBANK/AF408641
SI  - GENBANK/AF408642
SI  - GENBANK/AF408643
SI  - GENBANK/AF408644
SI  - GENBANK/AF408645
SI  - GENBANK/AF408646
SI  - GENBANK/AF408647
SI  - GENBANK/AF408648
SI  - GENBANK/AF408649
SI  - GENBANK/AF408650
SI  - GENBANK/AF408651
SI  - GENBANK/AF408652
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 4R7X1O2820 (Chlorine)
RN  - Q40Q9N063P (Acetic Acid)
RN  - TJ904HH8SN (Tetrachloroethylene)
RN  - WD06X94M2D (Vinyl Chloride)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Acetic Acid/metabolism
MH  - Anaerobiosis
MH  - Bacteria, Anaerobic/classification/genetics/growth & development/*metabolism
MH  - Biodegradation, Environmental
MH  - Bioreactors
MH  - Chlorine/*metabolism
MH  - DNA, Ribosomal/analysis
MH  - *Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Methanol/metabolism
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Tetrachloroethylene/*metabolism
MH  - Vinyl Chloride/*metabolism
EDAT- 2003/03/08 04:00
MHDA- 2003/05/03 05:00
CRDT- 2003/03/08 04:00
PHST- 2003/03/08 04:00 [pubmed]
PHST- 2003/05/03 05:00 [medline]
PHST- 2003/03/08 04:00 [entrez]
AID - 10.1099/mic.0.26018-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2003 Feb;149(Pt 2):459-469. doi: 10.1099/mic.0.26018-0.