PMID- 12626414
OWN - NLM
STAT- MEDLINE
DCOM- 20040112
LR  - 20191210
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 13
IP  - 4
DP  - 2003 Apr
TI  - Activities and expression pattern of the carbohydrate sulfotransferase 
      GlcNAc6ST-3 (I-GlcNAc6ST): functional implications.
PG  - 245-54
AB  - In recent years, a family of five GlcNAc-6-O-sulfotransferases, called the 
      GlcNAc6STs, has been molecularly cloned. One of these, GlcNAc6ST-2 (originally 
      named HEC-GlcNAc6ST or LSST), shows a very restricted expression at the mRNA 
      level in high endothelial cells (HECs) of lymph nodes high endothelial venules 
      (HEVs). This enzyme has been shown to be involved in elaborating the 6-sulfo sLex 
      structure on a set of mucin-like acceptors within HECs, thus providing a critical 
      recognition determinant for L-selectin during the process of lymphocyte homing to 
      lymph nodes. Limited information has been available about the closely related 
      sulfotransferase known as GlcNAc6ST-3 (I-GlcNAc6ST). Here, employing transfection 
      experiments with a series of glycoprotein acceptors, we report that this 
      sulfotransferase has a marked preference for sulfating O-linked sugars of 
      mucin-type acceptors, whereas other sulfotransferases in the family (GlcNAc6ST-1, 
      GlcNAc6ST-2) and a Gal-6-O-sulfotransferase exhibit strong activity on both 
      mucin-type acceptors and glycoproteins with predominantly N-linked chains. PCR 
      analysis of cDNAs derived from a panel of tissues and purified cell populations 
      confirms the strong expression of GlcNAc6ST-3 in gut-associated tissues and 
      extends the expression to include lymphocytes. In contrast to GlcNAc6ST-2, 
      GlcNAc6ST-3 transcripts are present minimally, if at all, in HECs; moreover, this 
      enzyme is not able to generate the 6-sulfo sLex epitope in transfected cells. 
      These latter findings argue that GlcNAc6ST-3 is not involved in generating 
      HEV-expressed ligands for L-selectin.
FAU - Lee, Jin Kyu
AU  - Lee JK
AD  - Department of Anatomy, University of California, San Francisco, CA 94143-0452, 
      USA.
FAU - Bistrup, Annette
AU  - Bistrup A
FAU - van Zante, Annemieke
AU  - van Zante A
FAU - Rosen, Steven D
AU  - Rosen SD
LA  - eng
GR  - R01GM5741/GM/NIGMS NIH HHS/United States
GR  - R37GM23547/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20021126
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Isoenzymes)
RN  - 0 (Ligands)
RN  - 0 (Oligosaccharides)
RN  - 0 (Sulfates)
RN  - 126880-86-2 (L-Selectin)
RN  - EC 2.8.2.- (Sulfotransferases)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - *Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Isoenzymes/genetics/metabolism
MH  - L-Selectin/metabolism
MH  - Ligands
MH  - Lymphatic System/enzymology
MH  - Male
MH  - Oligosaccharides/chemistry/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Substrate Specificity
MH  - Sulfates/metabolism
MH  - Sulfotransferases/*genetics/*metabolism
EDAT- 2003/03/11 04:00
MHDA- 2004/01/13 05:00
CRDT- 2003/03/11 04:00
PHST- 2003/03/11 04:00 [pubmed]
PHST- 2004/01/13 05:00 [medline]
PHST- 2003/03/11 04:00 [entrez]
AID - cwg018 [pii]
AID - 10.1093/glycob/cwg018 [doi]
PST - ppublish
SO  - Glycobiology. 2003 Apr;13(4):245-54. doi: 10.1093/glycob/cwg018. Epub 2002 Nov 
      26.