PMID- 12626447
OWN - NLM
STAT- MEDLINE
DCOM- 20031020
LR  - 20190508
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 10
IP  - 2
DP  - 2003 Mar
TI  - Detection of cross-reactivity for atopic immunoglobulin E against multiple 
      allergens.
PG  - 229-32
AB  - The existence of specific immunoglobulin E (IgE) allows us to determine the 
      allergens that cause the allergic disease. For the purposes of allergen avoidance 
      and immunotherapy, the measurement of specific IgE is widely applied in clinical 
      laboratories. However, if IgE from the serum of an allergic patient exhibits 
      reactivity to multiple allergens, it would cause a problem. The present study 
      analyzes whether the serum IgE with multiple reactivity is due to the presence of 
      unique IgE against the common epitope shared by different allergens or the 
      presence of multiple IgEs against different epitopes on different allergens. The 
      quantitative-competitive inhibition tests and the immunoblotting were applied to 
      analyze the immunosimilarity among examined allergens. The result shows that the 
      competitive inhibition of IgE binding between shrimp and crab allergens is higher 
      than those between either shrimp and cockroach or between crab and cockroach. 
      Furthermore, the results of immunoblotting are consistent with those of 
      quantitative-competitive inhibition tests. These results allow us to detect the 
      cross-reactivity for atopic IgE against multiple allergens.
FAU - Chiou, Yee-Hsuan
AU  - Chiou YH
AD  - Department of Pediatrics, Kaohsiung Veterans General Hospital and National 
      Yang-Ming University, Kaohsiung Hsien, Taiwan, Republic of China.
FAU - Yuo, Chung-Yee
AU  - Yuo CY
FAU - Wang, Lin-Yu
AU  - Wang LY
FAU - Huang, Shiao-ping
AU  - Huang SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Allergens)
RN  - 0 (Dust)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Brachyura
MH  - Cockroaches
MH  - Cross Reactions
MH  - Dogs
MH  - Dust
MH  - Humans
MH  - Hypersensitivity/epidemiology/*immunology
MH  - Immunoblotting
MH  - Immunoglobulin E/analysis/*immunology
MH  - Pandalidae
MH  - Pyroglyphidae
MH  - Seroepidemiologic Studies
MH  - Shellfish
PMC - PMC150535
EDAT- 2003/03/11 04:00
MHDA- 2003/10/21 05:00
PMCR- 2003/03/01
CRDT- 2003/03/11 04:00
PHST- 2003/03/11 04:00 [pubmed]
PHST- 2003/10/21 05:00 [medline]
PHST- 2003/03/11 04:00 [entrez]
PHST- 2003/03/01 00:00 [pmc-release]
AID - 0194 [pii]
AID - 10.1128/cdli.10.2.229-232.2003 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2003 Mar;10(2):229-32. doi: 
      10.1128/cdli.10.2.229-232.2003.