PMID- 12627171
OWN - NLM
STAT- MEDLINE
DCOM- 20030710
LR  - 20191210
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 21
IP  - 4
DP  - 2003 Apr
TI  - Site-specific gene targeting in mouse embryonic stem cells with intact bacterial 
      artificial chromosomes.
PG  - 447-51
AB  - Homologous recombination in Escherichia coli simplifies the generation of gene 
      targeting constructs for transduction into mouse embryonic stem (ES) cells. 
      Taking advantage of the extensive homology provided by intact bacterial 
      artificial chromosomes (BACs), we have developed an efficient method for 
      preparing targeted gene disruptions in ES cells. Correctly integrated clones were 
      identified by a simple screening procedure based on chromosomal fluorescence in 
      situ hybridization (FISH). To date, five mutant lines have been generated and 
      bred to homozygosity by this approach.
FAU - Yang, Yi
AU  - Yang Y
AD  - Department of Molecular Biology, Massachusetts General Hospital, and Department 
      of Genetics, Harvard Medical School, Boston, MA 02114, USA.
FAU - Seed, Brian
AU  - Seed B
LA  - eng
GR  - AI27849/AI/NIAID NIH HHS/United States
GR  - AI46731/AI/NIAID NIH HHS/United States
GR  - HL66678/HL/NHLBI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
DEP - 20030310
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Cloning, Molecular/*methods
MH  - Escherichia coli/genetics
MH  - Gene Targeting/*methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Mice
MH  - *Mutagenesis, Site-Directed
MH  - Polymerase Chain Reaction/methods
MH  - Recombination, Genetic/genetics
MH  - Stem Cells/physiology
MH  - Transduction, Genetic/*methods
EDAT- 2003/03/11 04:00
MHDA- 2003/07/11 05:00
CRDT- 2003/03/11 04:00
PHST- 2002/08/27 00:00 [received]
PHST- 2003/01/16 00:00 [accepted]
PHST- 2003/03/11 04:00 [pubmed]
PHST- 2003/07/11 05:00 [medline]
PHST- 2003/03/11 04:00 [entrez]
AID - nbt803 [pii]
AID - 10.1038/nbt803 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2003 Apr;21(4):447-51. doi: 10.1038/nbt803. Epub 2003 Mar 10.