PMID- 12628762
OWN - NLM
STAT- MEDLINE
DCOM- 20030501
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 92
IP  - 1-2
DP  - 2003 Mar 20
TI  - Sequencing and analysis of genomic DNA and cDNA encoding TNF-alpha in the 
      squirrel monkey (Saimiri sciureus).
PG  - 37-43
AB  - If a number of cytokines and growth factors that have been characterized from 
      human cells were investigated in non-human primates, results from such approaches 
      would allow the development of assays to detect and quantitate cytokines in 
      experimental models. Tumor necrosis factor-alpha (TNF-alpha) is an important 
      pluripotent cytokine which plays a crucial role in host defense. As yet, no 
      complete molecular data have been reported for the squirrel monkey TNF-alpha. 
      Polymerase chain reaction (PCR) primers were used to trace introns, by comparing 
      product sizes obtained using cDNA and genomic DNA as templates. The genomic DNA 
      is composed of four exons and three introns with 1793 nucleotides. The 
      corresponding cDNA is 702 nucleotides and phylogenetic analysis showed that the 
      Saimiri sciureus was most closely related to that of the genus Aotus, a new-world 
      primate, compared to old-world primates (genus Macaca and Papio). The deduced 
      TNF-alpha protein consists of 233 amino acids with 82% identity to human, 95% to 
      new-world monkeys and 79% to old-world monkeys. The cloned TNF-alpha cDNA will be 
      useful to quantitate TNF-alpha at the mRNA level.
FAU - Merien, Fabrice
AU  - Merien F
AD  - Centre de Primatologie, Institut Pasteur de la Guyane, BP 6010, 97306 Cayenne 
      Cedex, French Guiana. fmerien@pasteur-cayenne.fr
FAU - Lavergne, Anne
AU  - Lavergne A
FAU - Behr, Charlotte
AU  - Behr C
FAU - Contamin, Hugues
AU  - Contamin H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (DNA, Complementary)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary/chemistry/*genetics
MH  - Exons/genetics
MH  - Introns/genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Saimiri/*genetics/immunology
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2003/03/12 04:00
MHDA- 2003/05/02 05:00
CRDT- 2003/03/12 04:00
PHST- 2003/03/12 04:00 [pubmed]
PHST- 2003/05/02 05:00 [medline]
PHST- 2003/03/12 04:00 [entrez]
AID - S0165242703000187 [pii]
AID - 10.1016/s0165-2427(03)00018-7 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2003 Mar 20;92(1-2):37-43. doi: 
      10.1016/s0165-2427(03)00018-7.