PMID- 12628942
OWN - NLM
STAT- MEDLINE
DCOM- 20030324
LR  - 20190623
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 107
IP  - 9
DP  - 2003 Mar 11
TI  - Bone marrow-derived cardiomyocytes are present in adult human heart: A study of 
      gender-mismatched bone marrow transplantation patients.
PG  - 1247-9
AB  - BACKGROUND: Recent studies have identified cardiomyocytes of extracardiac origin 
      in transplanted human hearts, but the exact origin of these myocyte progenitors 
      is currently unknown. METHODS AND RESULTS: Hearts of female subjects (n=4) who 
      had undergone sex-mismatched bone marrow transplantation (BMT) were recovered at 
      autopsy and analyzed for the presence of Y chromosome-positive cardiomyocytes. 
      Four female gender-matched BMT subjects served as controls. Fluorescence in situ 
      hybridization (FISH) for the Y chromosome was performed on paraffin-embedded 
      sections to identify cells of bone marrow origin with concomitant 
      immunofluorescent labeling for alpha-sarcomeric actin to identify cardiomyocytes. 
      A total of 160 000 cardiomyocyte nuclei were analyzed approximating 20 000 nuclei 
      per patient. The mean percentage of Y chromosome-positive cardiomyocytes in 
      patients with sex-mismatched BMT was 0.23+/-0.06%. Not a single Y 
      chromosome-positive cardiomyocyte was identified in any of the control patients. 
      Immunofluorescent costaining for laminin and chromosomal ploidy analysis with 
      FISH showed no evidence of either pseudonuclei or cell fusion in any of the 
      chimeric cardiac myocytes identified. CONCLUSIONS: These data establish for the 
      first time human bone marrow as a source of extracardiac progenitor cells capable 
      of de novo cardiomyocyte formation.
FAU - Deb, Arjun
AU  - Deb A
AD  - Division of Cardiovascular Diseases and Molecular Medicine Program, Mayo Clinic, 
      Rochester, Minn 55905, USA.
FAU - Wang, Shaohua
AU  - Wang S
FAU - Skelding, Kimberly A
AU  - Skelding KA
FAU - Miller, Dylan
AU  - Miller D
FAU - Simper, David
AU  - Simper D
FAU - Caplice, Noel M
AU  - Caplice NM
LA  - eng
GR  - HL66958/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Actins)
SB  - IM
CIN - Circulation. 2003 Aug 26;108(8):e60; author reply e60. PMID: 12939249
MH  - Actins/analysis/immunology
MH  - Adult
MH  - *Bone Marrow Transplantation
MH  - Cell Lineage
MH  - Chromosomes, Human, Y
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Hematopoietic Stem Cells/*physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Myocardium/*cytology
MH  - Myocytes, Cardiac/chemistry/*cytology
MH  - Tissue Donors
MH  - Transplantation Chimera
EDAT- 2003/03/12 04:00
MHDA- 2003/03/26 04:00
CRDT- 2003/03/12 04:00
PHST- 2003/03/12 04:00 [pubmed]
PHST- 2003/03/26 04:00 [medline]
PHST- 2003/03/12 04:00 [entrez]
AID - 10.1161/01.cir.0000061910.39145.f0 [doi]
PST - ppublish
SO  - Circulation. 2003 Mar 11;107(9):1247-9. doi: 10.1161/01.cir.0000061910.39145.f0.