PMID- 12629219
OWN - NLM
STAT- MEDLINE
DCOM- 20030522
LR  - 20181130
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 100
IP  - 7
DP  - 2003 Apr 1
TI  - Essential function of alpha-calcium/calmodulin-dependent protein kinase II in 
      neurotransmitter release at a glutamatergic central synapse.
PG  - 4275-80
AB  - A significant fraction of the total calciumcalmodulin-dependent protein kinase II 
      (CaMKII) activity in neurons is associated with synaptic connections and is 
      present in nerve terminals, thus suggesting a role for CaMKII in neurotransmitter 
      release. To determine whether CaMKII regulates neurotransmitter release, we 
      generated and analyzed knockout mice in which the dominant alpha-isoform of 
      CaMKII was specifically deleted from the presynaptic side of the CA3-CA1 
      hippocampal synapse. Conditional CA3 alpha-CaMKII knockout mice exhibited an 
      unchanged basal probability of neurotransmitter release at CA3-CA1 synapses but 
      showed a significant enhancement in the activity-dependent increase in 
      probability of release during repetitive presynaptic stimulation, as was shown 
      with the analysis of unitary synaptic currents. These data indicate that 
      alpha-CaMKII serves as a negative activity-dependent regulator of 
      neurotransmitter release at hippocampal synapses and maintains synapses in an 
      optimal range of release probabilities necessary for normal synaptic operation.
FAU - Hinds, Heather L
AU  - Hinds HL
AD  - Howard Hughes Medical Institute, Picower Center for Learning and Memory, 
      RIKEN-MIT Neuroscience Research Center, Department of Biology, Massachusetts 
      Institute of Technology, Cambridge, MA 02139, USA.
FAU - Goussakov, Ivan
AU  - Goussakov I
FAU - Nakazawa, Kazu
AU  - Nakazawa K
FAU - Tonegawa, Susumu
AU  - Tonegawa S
FAU - Bolshakov, Vadim Y
AU  - Bolshakov VY
LA  - eng
GR  - R01 NS032925/NS/NINDS NIH HHS/United States
GR  - R01 NS044185/NS/NINDS NIH HHS/United States
GR  - R01 NS32925/NS/NINDS NIH HHS/United States
GR  - R01 NS44185/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030310
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Neurotransmitter Agents)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brain/enzymology/*physiology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/deficiency/*genetics/*physiology
MH  - Excitatory Postsynaptic Potentials
MH  - Glutamic Acid/*physiology
MH  - Hippocampus/*physiology
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neurotransmitter Agents/*metabolism
MH  - Restriction Mapping
MH  - Synapses/*physiology
PMC - PMC153083
EDAT- 2003/03/12 04:00
MHDA- 2003/05/23 05:00
PMCR- 2003/10/01
CRDT- 2003/03/12 04:00
PHST- 2003/03/12 04:00 [pubmed]
PHST- 2003/05/23 05:00 [medline]
PHST- 2003/03/12 04:00 [entrez]
PHST- 2003/10/01 00:00 [pmc-release]
AID - 0530202100 [pii]
AID - 0202 [pii]
AID - 10.1073/pnas.0530202100 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4275-80. doi: 
      10.1073/pnas.0530202100. Epub 2003 Mar 10.