PMID- 12630542
OWN - NLM
STAT- MEDLINE
DCOM- 20030924
LR  - 20190922
IS  - 0886-0440 (Print)
IS  - 0886-0440 (Linking)
VI  - 17
IP  - 5
DP  - 2002 Sep-Oct
TI  - Laser myocardial revascularization modulates expression of angiogenic, neuronal, 
      and inflammatory cytokines in a porcine model of chronic myocardial ischemia.
PG  - 413-24
AB  - BACKGROUND: Controversy exists whether transmyocardial laser revascularization 
      (TMR) is associated with angiogenesis or neuromodulation and whether these are 
      time-dependent phenomena. Accordingly, we performed a time-course analysis of the 
      expression of angiogenic and neuronal factors following experimental percutaneous 
      TMR. METHODS AND RESULTS: Five weeks after placing ameroid constrictors on the 
      circumflex coronary artery, 16 pigs underwent left ventricular mapping guided TMR 
      using Ho:YAG laser (2 J x 1 pulse) at 30 sites directed at the ischemic zones and 
      11 animals were ischemic controls. Histology and immunostaining were obtained at 
      1 and 2 weeks (4 TMR and 3 controls at each time point) and at 4 weeks (8 TMR and 
      5 controls) for vascular endothelial growth factor (VEGF), basic fibroblast 
      growth factor (bFGF), nerve growth factor (betaNGF), substance P (SP), and 
      monocyte chemoattractant protein-1 (MCP-1). Immunoreactivity was scored using a 
      digital image analysis system. Factor VIII staining was used for blood vessel 
      counting. Enhanced regional expression of VEGF, bFGF and MCP-1 in the TMR group 
      was noted at 1 and 2 weeks with a threefold increase at 4 weeks following TMR 
      compared to controls. BetaNGF expression in the TMR group was enhanced at 1 and 2 
      weeks with subsequent decline at 4 weeks to the controls level. SP expression was 
      not significantly different between groups at all time points. There was a 
      twofold increase in the number of blood vessels in the TMR group at 4 weeks, 
      which was not apparent earlier. CONCLUSIONS: These immunohistological findings 
      suggest that cytokines expression compatible with angiogenesis and 
      neuromodulation occurs early after TMR. Up-regulation of angiogenic and 
      inflammatory cytokines may be more sustained than neuromodulation.
FAU - Fuchs, Shmuel
AU  - Fuchs S
AD  - Cardiovascular Research Institute,Washington Hospital Center, Washington, DC 
      20010, USA. shmuel.fuchs@medstar.net
FAU - Baffour, Richard
AU  - Baffour R
FAU - Vodovotz, Yoram
AU  - Vodovotz Y
FAU - Shou, Matie
AU  - Shou M
FAU - Stabile, Eugenio
AU  - Stabile E
FAU - Tio, Fermin O
AU  - Tio FO
FAU - Leon, Martin B
AU  - Leon MB
FAU - Kornowski, Ran
AU  - Kornowski R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Card Surg
JT  - Journal of cardiac surgery
JID - 8908809
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Computer Graphics
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Fibroblast Growth Factor 2/metabolism
MH  - Immunohistochemistry
MH  - Laser Therapy/*methods
MH  - Myocardial Ischemia/*metabolism/pathology
MH  - Myocardial Revascularization/*methods
MH  - Neovascularization, Physiologic
MH  - Nerve Growth Factor/metabolism
MH  - Pilot Projects
MH  - Receptors, Vascular Endothelial Growth Factor/metabolism
MH  - Swine
EDAT- 2003/03/13 04:00
MHDA- 2003/09/25 05:00
CRDT- 2003/03/13 04:00
PHST- 2003/03/13 04:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/03/13 04:00 [entrez]
AID - 10.1111/j.1540-8191.2001.tb01171.x [doi]
PST - ppublish
SO  - J Card Surg. 2002 Sep-Oct;17(5):413-24. doi: 10.1111/j.1540-8191.2001.tb01171.x.