PMID- 12630901
OWN - NLM
STAT- MEDLINE
DCOM- 20031106
LR  - 20240109
IS  - 0885-4513 (Print)
IS  - 0885-4513 (Linking)
VI  - 37
IP  - Pt 2
DP  - 2003 Apr
TI  - Phytoremediation of 2,4-dichlorophenol by Brassica napus hairy root cultures.
PG  - 139-44
AB  - We have obtained hairy root cultures of Brassica napus with high biomass and 
      genetic stability which produce peroxidases, enzymes involved in biodegradation 
      processes. In this work, these hairy root cultures were used to study the removal 
      of 2,4-dichlorophenol (2,4-DCP), a common contaminant in industrial effluents 
      that is highly toxic for human and aquatic life. The optimum conditions to obtain 
      high efficiency in the removal process were established. Roots were able to 
      remove 2,4-DCP from aqueous solutions containing 100-1000 mg/l, in the presence 
      of H(2)O(2) concentrations ranging from 5 to 10 mM. After a short period of 
      incubation (15 min), high removal efficiencies were achieved (91-94%) and maximal 
      removal, of approx. 97-98%, was obtained with 1 h of reaction. High removal 
      efficiencies (93-95%) were observed in a broad pH range (pH 3-9), reaching 98-99% 
      in the range pH 4-8. Moreover, roots could be re-used, almost for six consecutive 
      cycles, to remove 2,4-DCP. The oxidation catalysed by peroxidases would be the 
      main mechanism involved in this process. The results suggest that these cultures 
      could be useful tools for phytoremediation.
FAU - Agostini, Elizabeth
AU  - Agostini E
AD  - Departamento de Biologia Molecular, FCEFQN, Universidad Nacional de Rio Cuarto, 
      Ruta 36 Km 601, CP 5800 Rio Cuarto, Cordoba, Argentina.
FAU - Coniglio, Maria S
AU  - Coniglio MS
FAU - Milrad, Silvia R
AU  - Milrad SR
FAU - Tigier, Horacio A
AU  - Tigier HA
FAU - Giulietti, Ana M
AU  - Giulietti AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (Chlorophenols)
RN  - 0 (Soil Pollutants)
RN  - 0 (Water Pollutants, Chemical)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Brassica napus/drug effects/enzymology/*metabolism
MH  - Chlorophenols/*pharmacokinetics
MH  - Culture Techniques
MH  - Environmental Pollution/*prevention & control
MH  - Hydrogen Peroxide/pharmacology
MH  - Peroxidase/*metabolism
MH  - Plant Roots/drug effects/enzymology/*metabolism
MH  - Soil Pollutants/metabolism
MH  - Water Pollutants, Chemical/metabolism
EDAT- 2003/03/13 04:00
MHDA- 2003/11/07 05:00
CRDT- 2003/03/13 04:00
PHST- 2003/03/13 04:00 [pubmed]
PHST- 2003/11/07 05:00 [medline]
PHST- 2003/03/13 04:00 [entrez]
AID - 10.1042/ba20020079 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2003 Apr;37(Pt 2):139-44. doi: 10.1042/ba20020079.