PMID- 12632067
OWN - NLM
STAT- MEDLINE
DCOM- 20031117
LR  - 20061115
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 22
IP  - 4
DP  - 2003 Apr
TI  - Monocyte chemoattractant protein-1 expression correlates with macrophage 
      infiltration and tumor vascularity in human gastric carcinomas.
PG  - 773-8
AB  - The purpose of this study was to investigate the role of interactions between 
      tumor cells and macrophages during angiogenesis in human gastric carcinomas. 
      Macrophage infiltration into tumors and monocyte chemoattractant protein-1 
      (MCP-1) expression was assessed in 72 archival specimens of gastric carcinoma for 
      comparison with tumor vascularity. The mRNA expression of MCP-1 was examined by 
      RT-PCR in 6 gastric carcinoma cell lines and in fresh biopsy specimens from 18 
      patients. Immunolocalization of representative angiogenic factors, vascular 
      endothelial growth factor (VEGF), and platelet-derived endothelial cell growth 
      factor (PD-ECGF) was also done. MCP-1 expression in tumor cells increased with 
      the depth of tumor invasion (Tis 9.5%, T1 19.4%, T2-4 60.0%), as did microvessel 
      density and macrophage infiltration. Macrophage counts correlated with vessel 
      counts, and both were significantly higher in MCP-1-positive than in negative 
      tumors. Of the 6 gastric carcinoma cell lines, 2 constitutively expressed MCP-1 
      mRNA. In 6 (33.3%) of 18 biopsy samples, MCP-1 mRNA was expressed at higher 
      levels in tumor tissues than in normal mucosa. VEGF protein was expressed by 
      gastric carcinoma cells, whereas PD-ECGF protein was expressed mainly by stromal 
      mononuclear cells. MCP-1 expression correlated significantly with VEGF but not 
      PD-ECGF expression in gastric carcinomas. These results suggest that MCP-1 
      produced by human gastric carcinoma cells plays a role in angiogenesis via 
      macrophage recruitment and activation.
FAU - Ohta, Masahiro
AU  - Ohta M
AD  - Department of Medicine and Molecular Science, Hiroshima University, Hiroshima, 
      Japan.
FAU - Kitadai, Yasuhiko
AU  - Kitadai Y
FAU - Tanaka, Shinji
AU  - Tanaka S
FAU - Yoshihara, Masaharu
AU  - Yoshihara M
FAU - Yasui, Wataru
AU  - Yasui W
FAU - Mukaida, Naofumi
AU  - Mukaida N
FAU - Haruma, Ken
AU  - Haruma K
FAU - Chayama, Kazuaki
AU  - Chayama K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.4.2.4 (Thymidine Phosphorylase)
SB  - IM
MH  - Carcinoma/blood supply/*metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - Macrophages/*metabolism
MH  - Neoplasm Invasiveness
MH  - Neovascularization, Pathologic
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stomach Neoplasms/blood supply/*metabolism/*pathology
MH  - Thymidine Phosphorylase/biosynthesis
MH  - Vascular Endothelial Growth Factor A/biosynthesis
EDAT- 2003/03/13 04:00
MHDA- 2003/12/03 05:00
CRDT- 2003/03/13 04:00
PHST- 2003/03/13 04:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/03/13 04:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2003 Apr;22(4):773-8.