PMID- 12632312
OWN - NLM
STAT- MEDLINE
DCOM- 20030605
LR  - 20160526
IS  - 0743-684X (Print)
IS  - 0743-684X (Linking)
VI  - 19
IP  - 2
DP  - 2003 Feb
TI  - Schwann-cell proliferation in muscle-vein combined conduits for bridging rat 
      sciatic nerve defects.
PG  - 119-23; discussion 124
AB  - Among the various grafting procedures that have been studied as alternatives to 
      traditional fresh nerve autografts for the repair of severed peripheral nerves, 
      muscle-vein-combined graft conduits have recently been devised and successfully 
      employed. In the present study, the early presence, origin, and proliferation 
      activity of Schwann cells (SCs) along this particular type of biological graft 
      conduit have been investigated, using antibodies directed against glial fibrillar 
      acid protein (GFAP), a protein that is specifically expressed in glial cells, and 
      proliferating cell nuclear antigen (PCNA), a protein that is expressed by cells 
      during DNA synthesis. Results showed that the muscle-vein-combined graft was 
      progressively invaded by a number of GFAP-immunopositive SCs, many of which were 
      also found to be immunopositive for PCNA, thus demonstrating that their 
      proliferation continues to occur inside the graft. Among the molecules that could 
      be involved in the stimulation of Schwann-cell proliferation is neuregulin-1 
      (NRG-1) that mediates its effects by binding to the ErbB receptor tyrosine kinase 
      family. In the present study, the authors report on the RT-PCR analysis for NRG-1 
      and ErbB3 mRNAs, showing an overall increase in the content of these transcripts 
      inside the muscle-vein-combined graft. These results suggest that the 
      muscle-vein-combined graft conduit constitutes an environment favorable to 
      potentiate Schwann-cell proliferation during the early regeneration phases.
FAU - Geuna, S
AU  - Geuna S
AD  - Department of Clinical and Biologic Sciences, University of Turin, Ospedale San 
      Luigi, Italy.
FAU - Raimondo, S
AU  - Raimondo S
FAU - Nicolino, S
AU  - Nicolino S
FAU - Boux, E
AU  - Boux E
FAU - Fornaro, M
AU  - Fornaro M
FAU - Tos, P
AU  - Tos P
FAU - Battiston, B
AU  - Battiston B
FAU - Perroteau, I
AU  - Perroteau I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Reconstr Microsurg
JT  - Journal of reconstructive microsurgery
JID - 8502670
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Division
MH  - Cell Movement/physiology
MH  - Cell Transplantation
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Confocal
MH  - Microsurgery/methods
MH  - Molecular Sequence Data
MH  - Muscles/transplantation
MH  - Nerve Regeneration/physiology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Schwann Cells/*transplantation
MH  - Sciatic Nerve/*pathology/*surgery
MH  - Sensitivity and Specificity
MH  - Tissue Transplantation/methods
MH  - Veins/transplantation
EDAT- 2003/03/13 04:00
MHDA- 2003/06/06 05:00
CRDT- 2003/03/13 04:00
PHST- 2003/03/13 04:00 [pubmed]
PHST- 2003/06/06 05:00 [medline]
PHST- 2003/03/13 04:00 [entrez]
AID - 10.1055/s-2003-37818 [doi]
PST - ppublish
SO  - J Reconstr Microsurg. 2003 Feb;19(2):119-23; discussion 124. doi: 
      10.1055/s-2003-37818.