PMID- 12632314
OWN - NLM
STAT- MEDLINE
DCOM- 20030605
LR  - 20181130
IS  - 0743-684X (Print)
IS  - 0743-684X (Linking)
VI  - 19
IP  - 2
DP  - 2003 Feb
TI  - Enhanced peripheral nerve regeneration elicited by cell-mediated events delivered 
      via a bioresorbable PLGA guide.
PG  - 125-34
AB  - Using an established rat peripheral-nerve regeneration model, the authors have 
      demonstrated enhancement of regeneration following subcutaneous priming of 
      bioresorbable poly(lactic-co-glycolic)acid (PLGA) guides in vivo. Four weeks 
      after nerve reconstruction, regeneration of the peripheral nerve through the 
      cell-infiltrated guides displayed a significant increase in the total axon number 
      and myelination status recorded in primed over unprimed guides, demonstrating the 
      importance of cell-mediated events in the regeneration process. To define the 
      different components enhancing nerve regeneration in this model, they have 
      focused on identifying factors capable of eliciting Schwann-cell migration, since 
      this has been identified as an early and necessary event in nerve regeneration. 
      Using an in vitro migration assay, screening of a limited number of cellular and 
      extracellular factors has demonstrated differential promotion of Schwann-cell 
      migration. Of interest, combining fibronectin and bFGF resulted in a two-fold 
      enhancement in Schwann-cell migration over that recorded with either alone. These 
      results describe a rapid screening process for identifying various molecules and 
      combinations thereof, with potential involvement in Schwann-cell migration. 
      Coupling these findings to the use of the PLGA guide as an in vivo delivery 
      system provides a rationale for the selection of exogenous factors to test for 
      the enhancement of peripheral-nerve regeneration.
FAU - Bryan, David J
AU  - Bryan DJ
AD  - Tissue Engineering Laboratory, Research and Education Institute, Lahey Clinic, 
      Burlington, Massachusetts 01805, USA.
FAU - Tang, Jin Bo
AU  - Tang JB
FAU - Holway, Antonia H
AU  - Holway AH
FAU - Rieger-Christ, Kimberly M
AU  - Rieger-Christ KM
FAU - Trantolo, Debra J
AU  - Trantolo DJ
FAU - Wise, Donald L
AU  - Wise DL
FAU - Summerhayes, Ian C
AU  - Summerhayes IC
LA  - eng
GR  - R01DK59400/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Reconstr Microsurg
JT  - Journal of reconstructive microsurgery
JID - 8502670
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - Cell Movement
MH  - Disease Models, Animal
MH  - Female
MH  - Immunohistochemistry
MH  - Lactic Acid/*pharmacology
MH  - Male
MH  - Nerve Regeneration/*physiology
MH  - Peripheral Nerves/*pathology
MH  - Polyglycolic Acid/*pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*pharmacology
MH  - Probability
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reference Values
MH  - Schwann Cells/*transplantation
MH  - Sciatic Nerve/*physiology/*surgery
MH  - Sensitivity and Specificity
EDAT- 2003/03/13 04:00
MHDA- 2003/06/06 05:00
CRDT- 2003/03/13 04:00
PHST- 2003/03/13 04:00 [pubmed]
PHST- 2003/06/06 05:00 [medline]
PHST- 2003/03/13 04:00 [entrez]
AID - 10.1055/s-2003-37820 [doi]
PST - ppublish
SO  - J Reconstr Microsurg. 2003 Feb;19(2):125-34. doi: 10.1055/s-2003-37820.