PMID- 12633869
OWN - NLM
STAT- MEDLINE
DCOM- 20030425
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 538
IP  - 1-3
DP  - 2003 Mar 13
TI  - Nucleoporation of dendritic cells: efficient gene transfer by electroporation 
      into human monocyte-derived dendritic cells.
PG  - 149-54
AB  - Dendritic cells (DCs) are ideal accessory cells in the developing field of gene 
      therapy. Although viral transfection of DCs has become widespread, non-viral 
      transfection of DCs has shown disappointing results. Recently, a new technique 
      for transfecting primary cells has become available -- the Amaxa Nucleofector. 
      Here, we describe the use of this device in the successful non-viral transfection 
      of human monocyte-derived DCs. Using enhanced green fluorescent protein as a 
      reporter gene DCs were transfectable with efficiencies approaching 60%, remaining 
      responsive to lipopolysaccharide-stimulated cytokine production in short-term 
      experiments (though long-term functional assays were hampered by loss of 
      viability). Although these data demonstrate the ease and efficiency with which 
      human monocyte-derived DCs can now be non-virally transfected, they also suggest 
      the limitations of this technology due to the gradual loss of cell viability. The 
      potential use of this system in the development of DC-based cell and gene 
      therapies will be hampered until cell viability can be maintained.
FAU - Lenz, Petra
AU  - Lenz P
AD  - Laboratory of Cellular Oncology, National Cancer Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Bacot, Silvia M
AU  - Bacot SM
FAU - Frazier-Jessen, Michelle R
AU  - Frazier-Jessen MR
FAU - Feldman, Gerald M
AU  - Feldman GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Dendritic Cells/immunology/*metabolism
MH  - *Electroporation
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Immunophenotyping
MH  - Kinetics
MH  - Luminescent Proteins/metabolism
MH  - Monocytes/immunology/*metabolism
MH  - Transfection
EDAT- 2003/03/14 04:00
MHDA- 2003/04/26 05:00
CRDT- 2003/03/14 04:00
PHST- 2003/03/14 04:00 [pubmed]
PHST- 2003/04/26 05:00 [medline]
PHST- 2003/03/14 04:00 [entrez]
AID - S0014579303001698 [pii]
AID - 10.1016/s0014-5793(03)00169-8 [doi]
PST - ppublish
SO  - FEBS Lett. 2003 Mar 13;538(1-3):149-54. doi: 10.1016/s0014-5793(03)00169-8.