PMID- 12636175
OWN - NLM
STAT- MEDLINE
DCOM- 20030909
LR  - 20190818
IS  - 0724-8741 (Print)
IS  - 0724-8741 (Linking)
VI  - 20
IP  - 2
DP  - 2003 Feb
TI  - Gene expression profiles of ABC transporters and cytochrome P450 3A in Caco-2 and 
      human colorectal cancer cell lines.
PG  - 324-7
AB  - PURPOSE: The mRNA levels of MDR1 (P-glycoprotein), multidrug 
      resistance-associated proteins (MRP1, MRP2), cytochrome P450 3A (CYP3A) and 
      villin in human colorectal cell lines (HCT-15, LoVo DLD-1, HCT-116 and SW620) 
      were quantitatively compared with those in Caco-2 cells. METHODS: The mRNA levels 
      were determined by real time quantitative polymerase chain reaction and expressed 
      as the relative concen trations of MDR1 mRNA to glyceraldehyde-3-phosphate 
      dehydro genase (GAPDH) mRNA. RESULTSl MDR1 mRNA was expressed in HCT-15 LoVo and 
      DLD-1 cells at similar or lower level to Caco-2. The expression of MRP mRNA in 
      the cell lines tested was comparable with Caco-2. MRP. mRNA was detected only in 
      HCT-116 and SW620 at significantly lower level than Caco-2. CYP3A mRNA was 
      detected in HCT-15 LoVo, DLD-1 and SW620 at similar level to Caco-2. CONCLUSIONS: 
      HCT-15 LoVo and DLD-1 cells express proteins important for regulating the 
      intestinal absorption of drugs, i.e., MDR1 MRP1 and CYP3A, whereas HCT-116 and 
      SW620 cells were not acceptable for evaluation of absorption properties of drug 
      candidates
FAU - Nakumura, Tsutomu
AU  - Nakumura T
AD  - Department of Hospital Pharmacy, School of Medicine, Kobe University, Chuo-ku, 
      Kobe 650-0017, Japan.
FAU - Sakaeda, Toshiyuki
AU  - Sakaeda T
FAU - Ohmoto, Nobuko
AU  - Ohmoto N
FAU - Moriya, Yuka
AU  - Moriya Y
FAU - Komoto, Chiho
AU  - Komoto C
FAU - Shirakawa, Toshiro
AU  - Shirakawa T
FAU - Gotoh, Akinobu
AU  - Gotoh A
FAU - Matsuo, Masafumi
AU  - Matsuo M
FAU - Okmura, Katsuhiko
AU  - Okmura K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - EC 1.5.- (Oxidoreductases, N-Demethylating)
SB  - IM
MH  - ATP-Binding Cassette Transporters/*biosynthesis/genetics
MH  - Aryl Hydrocarbon Hydroxylases/*biosynthesis/genetics
MH  - Caco-2 Cells
MH  - Colorectal Neoplasms/enzymology/*metabolism
MH  - Cytochrome P-450 CYP3A
MH  - *Gene Expression Profiling/methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Oxidoreductases, N-Demethylating/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Tumor Cells, Cultured
EDAT- 2003/03/15 04:00
MHDA- 2003/09/10 05:00
CRDT- 2003/03/15 04:00
PHST- 2003/03/15 04:00 [pubmed]
PHST- 2003/09/10 05:00 [medline]
PHST- 2003/03/15 04:00 [entrez]
AID - 10.1023/a:1022251910820 [doi]
PST - ppublish
SO  - Pharm Res. 2003 Feb;20(2):324-7. doi: 10.1023/a:1022251910820.