PMID- 12637264
OWN - NLM
STAT- MEDLINE
DCOM- 20030722
LR  - 20200930
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 285
IP  - 1
DP  - 2003 Jul
TI  - High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 
      monocytic cell rolling on endothelium.
PG  - C112-8
AB  - Leukocyte rolling, adhesion, and migration on vascular endothelium involve 
      several sets of adhesion molecules that interact simultaneously. Each of these 
      receptor-ligand pairs may play multiple roles. We examined the role of ICAM-1 in 
      adhesive interactions with mouse aortic endothelial cells (MAECs) in an in vitro 
      flow system. Average rolling velocity of the monocytic cell line WEHI 274.1 was 
      increased on ICAM-1-deficient MAECs compared with wild-type MAECs, both with and 
      without TNF-alpha stimulation. High-temporal-resolution analysis provided 
      insights into the underlying basis for these differences. Without TNF-alpha 
      stimulation, average rolling velocity was slower on wild-type than on 
      ICAM-1-deficient endothelium because of brief (<1 s) pauses. On 
      TNF-alpha-stimulated ICAM-1-deficient endothelium, cells rolled faster because of 
      transient accelerations, producing "jerky" rolling. Firm adhesion to 
      ICAM-1-deficient MAECs was significantly reduced compared with wild-type MAECs, 
      although the number of rolling cells was similar. These results demonstrate 
      directly that ICAM-1 affects rolling velocity by stabilizing leukocyte rolling.
FAU - Kevil, Christopher G
AU  - Kevil CG
AD  - Department of Genomics and Pathobiology, University of Alabama at Birmingham, 
      Birmingham, AL 35294, USA.
FAU - Chidlow, John H
AU  - Chidlow JH
FAU - Bullard, Daniel C
AU  - Bullard DC
FAU - Kucik, Dennis F
AU  - Kucik DF
LA  - eng
GR  - AR-46404/AR/NIAMS NIH HHS/United States
GR  - HL-10312/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030312
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Antineoplastic Agents)
RN  - 0 (P-Selectin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Aorta/cytology
MH  - Cell Adhesion/immunology
MH  - Cell Communication/immunology
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*cytology/drug effects/*metabolism
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Microscopy, Video
MH  - Monocytes/*cytology/*metabolism
MH  - P-Selectin/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2003/03/15 04:00
MHDA- 2003/07/23 05:00
CRDT- 2003/03/15 04:00
PHST- 2003/03/15 04:00 [pubmed]
PHST- 2003/07/23 05:00 [medline]
PHST- 2003/03/15 04:00 [entrez]
AID - 00334.2002 [pii]
AID - 10.1152/ajpcell.00334.2002 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2003 Jul;285(1):C112-8. doi: 
      10.1152/ajpcell.00334.2002. Epub 2003 Mar 12.