PMID- 12637633
OWN - NLM
STAT- MEDLINE
DCOM- 20031125
LR  - 20190513
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 18
IP  - 4
DP  - 2003 Apr
TI  - Increased expression of monocyte chemoattractant protein-1 (MCP-1) by renal 
      epithelial cells in culture on exposure to calcium oxalate, phosphate and uric 
      acid crystals.
PG  - 664-9
AB  - BACKGROUND: During the development of non-infectious kidney stones, crystals form 
      and deposit in the kidneys and become surrounded by monocytes/macrophages (M/M). 
      We have proposed that in response to crystal exposure renal epithelial cells 
      produce chemokines, which attract the M/M to the sites of crystal deposition. We 
      investigated the expression of monocyte chemoattractant protein-1 (MCP-1) mRNA 
      and protein by NRK52E rat renal tubular epithelial cells exposed to calcium 
      oxalate (CaOx), brushite (Br, a calcium phosphate) and uric acid (UA) crystals. 
      METHODS: Confluent cultures of NRK52E cells were exposed to CaOx, Br or UA at a 
      concentration of 250 micro g/ml (66.7 micro g/cm(2)). They were exposed for 1, 3, 
      6, 12, 24 and 48 h for isolation of mRNA and 24 h for ELISA to determine the 
      secretion of protein into the culture medium. Since cells are known to produce 
      free radicals on exposure to CaOx crystals we also investigated the effect of 
      free radical scavenger catalase on the crystal induced expression of MCP-1 mRNA 
      and protein. RESULTS: Exposure of NRK52E cells to the crystals resulted in 
      increased expression of MCP-1 mRNA and production of the chemoattractant. CaOx 
      crystals were most provocative while UA the least. Treatment with catalase had a 
      negative effect on the increased expression of both MCP-1 mRNA and protein, which 
      indicates the involvement of free radicals in up-regulation of MCP-1 production. 
      CONCLUSION: Exposure to both CaOx and calcium phosphate crystals stimulates 
      increased production of MCP-1. Free radicals appear to be involved in this 
      up-regulation. Results indicate that MCP-1, which is often associated with 
      localized inflammation, may be one of the chemokine mediators associated with the 
      deposition of various urinary crystals in the kidneys during kidney stone 
      formation. Because of the small number of experiments performed here, results 
      must be confirmed by more extensive studies with larger sample size.
FAU - Umekawa, Tohru
AU  - Umekawa T
AD  - Department of Pathology and Laboratory Medicine, University of Florida College of 
      Medicine, Gainesville, FL 32610-0275, USA.
FAU - Chegini, Nasser
AU  - Chegini N
FAU - Khan, Saeed R
AU  - Khan SR
LA  - eng
GR  - DK59765/DK/NIDDK NIH HHS/United States
GR  - R01 DK53962/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Biomarkers)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Chemokine CCL2)
RN  - 0 (alpha-tricalcium phosphate)
RN  - 0 (tetracalcium phosphate)
RN  - 2612HC57YE (Calcium Oxalate)
RN  - 268B43MJ25 (Uric Acid)
RN  - 701EKV9RMN (calcium phosphate, monobasic, anhydrous)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - L11K75P92J (calcium phosphate, dibasic, anhydrous)
SB  - IM
MH  - Base Sequence
MH  - Biomarkers/analysis
MH  - Calcium Oxalate/analysis/*metabolism
MH  - Calcium Phosphates/analysis/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/*metabolism
MH  - Crystallization
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells
MH  - Humans
MH  - Kidney/cytology
MH  - Kidney Calculi/*diagnosis/etiology
MH  - Molecular Sequence Data
MH  - Probability
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Statistics, Nonparametric
MH  - Uric Acid/*analysis
EDAT- 2003/03/15 04:00
MHDA- 2003/12/03 05:00
CRDT- 2003/03/15 04:00
PHST- 2003/03/15 04:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/03/15 04:00 [entrez]
AID - 10.1093/ndt/gfg140 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2003 Apr;18(4):664-9. doi: 10.1093/ndt/gfg140.