PMID- 12637753
OWN - NLM
STAT- MEDLINE
DCOM- 20030619
LR  - 20170214
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 40
IP  - 2
DP  - 2003 Mar
TI  - Detection rates of porcine reproductive and respiratory syndrome virus, porcine 
      circovirus type 2, and swine influenza virus in porcine proliferative and 
      necrotizing pneumonia.
PG  - 143-8
AB  - A retrospective study on pig lung tissues from 60 cases of proliferative and 
      necrotizing pneumonia (PNP) was performed to determine the presence of porcine 
      reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), 
      and porcine circovirus type 2 (PCV2) in these lesions. Cases selected included 30 
      cases diagnosed between 1988 and 1992 and 30 cases diagnosed between 1997 and 
      2001. In each group of 30 cases, 10 were from suckling piglets, whereas the other 
      20 were from postweaned animals representing either nursery or grower-finisher 
      pigs. Immunohistochemistry using a monoclonal antibody to influenza virus type A 
      was used to determine the presence of SIV, and in situ hybridization was used for 
      the detection of PRRSV and PCV2 nucleic acids. PRRSV was detected in 55 of the 60 
      cases examined (92%), PCV2 in 25 cases (42%), and SIV in only 1 case (2%). In 30 
      cases (50%), PRRSV was the only virus detected, whereas in 25 other cases (42%), 
      a combination of PRRSV and PCV2 could be detected in the lungs with PNP lesions. 
      PCV2 could not be detected in the lungs of suckling pigs with PNP. All 
      PCV2-positive cases were found in postweaned pigs and were always in combination 
      with PRRSV. In this latter age group, PCV2 was detected in 63% of the cases 
      (25/40). Data from our study indicate that SIV is rarely identified in PNP and 
      that PCV2 infection is not essential for the development of PNP lesions. The 
      results of the present study demonstrate that PRRSV is consistently and 
      predominantly associated with PNP and should be considered the key etiologic 
      agent for the condition.
FAU - Drolet, R
AU  - Drolet R
AD  - Departement de pathologie et de microbiologie, Faculte de medecine veterinaire, 
      Universite de Montreal, St-Hyacinthe, Quebec, Canada. 
      droletr@medvet.umontreal.ca.
FAU - Larochelle, R
AU  - Larochelle R
FAU - Morin, M
AU  - Morin M
FAU - Delisle, B
AU  - Delisle B
FAU - Magar, R
AU  - Magar R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Animals, Suckling
MH  - Circoviridae Infections/complications/veterinary/virology
MH  - Circovirus/*isolation & purification
MH  - Immunohistochemistry/veterinary
MH  - In Situ Hybridization/veterinary
MH  - Influenza A virus/*isolation & purification
MH  - Lung/virology
MH  - Orthomyxoviridae Infections/complications/veterinary/virology
MH  - Pneumonia, Viral/*veterinary/virology
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Retrospective Studies
MH  - Swine
EDAT- 2003/03/15 04:00
MHDA- 2003/06/20 05:00
CRDT- 2003/03/15 04:00
PHST- 2003/03/15 04:00 [pubmed]
PHST- 2003/06/20 05:00 [medline]
PHST- 2003/03/15 04:00 [entrez]
AID - 40/2/143 [pii]
AID - 10.1354/vp.40-2-143 [doi]
PST - ppublish
SO  - Vet Pathol. 2003 Mar;40(2):143-8. doi: 10.1354/vp.40-2-143.