PMID- 12637758
OWN - NLM
STAT- MEDLINE
DCOM- 20030619
LR  - 20170214
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 40
IP  - 2
DP  - 2003 Mar
TI  - Expression of monocyte chemoattractant protein-1 but not interleukin-8 in 
      granulomatous lesions in lymph nodes from pigs with naturally occurring 
      postweaning multisystemic wasting syndrome.
PG  - 181-6
AB  - Monocyte chemoattractant protein-1 (MCP-1) but not interleukin-8 (IL-8) was 
      detected by in situ hybridization using a nonradioactive digoxigenin-labeled 
      complementary DNA probe in granulomatous lesions of lymph nodes from 20 pigs with 
      naturally occurring postweaning multisystemic wasting syndrome (PMWS). 
      Complementary DNA probes of 375 and 266 base pairs for MCP-1 and IL-8, 
      respectively, were generated by reverse transcription-polymerase chain reaction. 
      The 20 pigs with PMWS had distinct positive hybridization signals for MCP-1 but 
      not for IL-8. The hybridization signals for MCP-1 were strictly confined to the 
      cells with granulomatous lesions, including macrophages and multinucleated giant 
      cells. A very close cell-to-cell correlation between MCP-1 and porcine circovirus 
      2 was seen in serial sections of lymph nodes. Results of this study indicate that 
      MCP-1 expression may play a role in the pathogenesis of granulomatous 
      inflammation in pigs with PMWS.
FAU - Kim, J
AU  - Kim J
AD  - Department of Veterinary Pathology, College of Veterinary Medicine asnd School of 
      Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Circoviridae Infections/metabolism/pathology/veterinary/virology
MH  - Circovirus/isolation & purification
MH  - Granuloma/*metabolism/pathology/virology
MH  - In Situ Hybridization/veterinary
MH  - Interleukin-8/*biosynthesis/genetics
MH  - Lymph Nodes/metabolism/*pathology
MH  - RNA, Viral/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Swine Diseases/*metabolism/pathology/virology
MH  - Wasting Syndrome/metabolism/pathology/*veterinary/virology
EDAT- 2003/03/15 04:00
MHDA- 2003/06/20 05:00
CRDT- 2003/03/15 04:00
PHST- 2003/03/15 04:00 [pubmed]
PHST- 2003/06/20 05:00 [medline]
PHST- 2003/03/15 04:00 [entrez]
AID - 40/2/181 [pii]
AID - 10.1354/vp.40-2-181 [doi]
PST - ppublish
SO  - Vet Pathol. 2003 Mar;40(2):181-6. doi: 10.1354/vp.40-2-181.