PMID- 12642404
OWN - NLM
STAT- MEDLINE
DCOM- 20031015
LR  - 20181113
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 138
IP  - 5
DP  - 2003 Mar
TI  - Expression and function of P2X purinoceptors in rat histaminergic neurons.
PG  - 1013-9
AB  - (1) The pharmacology of ATP responses and the expression pattern of seven known 
      subunits of the P2X receptor were investigated in individual histaminergic 
      neurons of the tuberomamillary nucleus (TM). (2) ATP (3-1000 micro M) evoked fast 
      non-desensitizing inward currents in TM neurons. 2-methylthioATP (2MeSATP) 
      displayed the same efficacy but a lower potency, EC(50)s 84 micro M versus 48 
      micro M, when compared with ATP. Adenosine-diphosphate (ADP), 
      uridine-triphosphate (UTP) and alpha beta methylene-ATP (alphabeta-meATP) were 
      inactive. (3) ATP-mediated whole cell currents were potentiated by acidification 
      of the recording solution (pH 7.5 and 6.6 were compared). (4) Single-cell RT-PCR 
      (scRT-PCR) analysis revealed that the P2X(2) receptor is expressed in all 
      PCR-positive neurons. Each of the P2X(1), P2X(3), P2X(4), P2X(5) and P2X(6) mRNAs 
      were detected in less than 35% of the cells. (5) Suramin antagonized ATP 
      responses with an IC(50) of 4.2 micro M and 
      pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 1 micro M) reduced 
      ATP responses to 43% of control, when antagonists were pre-applied 90s before the 
      agonist. Cibacron blue (3 micro M) given together with ATP potentiated control 
      responses by 67%, but inhibited it to 10% after pre-application. (6) 
      2',3'-O-(2,4,6-Trinitrophenyl) adenosine 5'-triphosphate (TNP-ATP) antagonized 
      ATP responses with an IC(50) of 7 micro M. (7) Pharmacological properties of ATP 
      responses together with scRT-PCR data suggest that P2X(2) is the major 
      purinoceptor on the soma of TM neurons, however the presence of heteromeric 
      P2X(2/5) receptors in some neurons cannot be excluded.
FAU - Vorobjev, Vladimir S
AU  - Vorobjev VS
AD  - Department of Neurophysiology, Heinrich-Heine-Universitat, POB 101007, D-40001 
      Dusseldorf, Germany.
FAU - Sharonova, Irina N
AU  - Sharonova IN
FAU - Haas, Helmut L
AU  - Haas HL
FAU - Sergeeva, Olga A
AU  - Sergeeva OA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Histamine Agents)
RN  - 0 (Purinergic P2 Receptor Agonists)
RN  - 0 (Receptors, Purinergic P2)
RN  - 820484N8I3 (Histamine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/*physiology
MH  - Histamine/*physiology
MH  - Histamine Agents/pharmacology
MH  - Male
MH  - Neurons/drug effects/*metabolism
MH  - Purinergic P2 Receptor Agonists
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Purinergic P2/*biosynthesis/genetics/*physiology
PMC - PMC1573743
EDAT- 2003/03/19 04:00
MHDA- 2003/10/16 05:00
PMCR- 2004/03/01
CRDT- 2003/03/19 04:00
PHST- 2003/03/19 04:00 [pubmed]
PHST- 2003/10/16 05:00 [medline]
PHST- 2003/03/19 04:00 [entrez]
PHST- 2004/03/01 00:00 [pmc-release]
AID - 0705144 [pii]
AID - 10.1038/sj.bjp.0705144 [doi]
PST - ppublish
SO  - Br J Pharmacol. 2003 Mar;138(5):1013-9. doi: 10.1038/sj.bjp.0705144.