PMID- 12644856
OWN - NLM
STAT- MEDLINE
DCOM- 20030508
LR  - 20111117
IS  - 1525-7886 (Print)
IS  - 1525-7886 (Linking)
VI  - 54
IP  - 1
DP  - 2003 Jan-Feb
TI  - Analysis of DNA in hair fibers.
PG  - 21-7
AB  - The extraction and identification of deoxyribonucleic acid (DNA) from human hair 
      shafts is described, along with the effects of hair treatments on levels of DNA 
      and suggestions of DNA location within the shaft. DNA was present at low levels 
      in the hair shaft, and was identified using polymerase chain reaction 
      amplification of the human leukocyte antigen (HLA)-DQA1 locus. The use of cleanup 
      columns aided the success of PCR amplification. DNA appears to reside in the 
      cuticle portion of the hair shaft. Levels of DNA were found to be higher at the 
      root-end compared to the tip-end of hair and were also found to be lower after 
      permanent colorant treatment. DNA was found to be lost with surfactant washing, 
      with increased loss occurring with prolonged or an increasing number of washes. 
      These results suggest that small amounts of residual DNA remain after 
      differentiation and add to our knowledge of the constituents of hair.
FAU - Heywood, Daniella M
AU  - Heywood DM
AD  - Hair Group, Unilever Research, Port Sunlight, Quarry Road East, Bebington, 
      Wirral, CH63 3JW, United Kingdom.
FAU - Skinner, Richard
AU  - Skinner R
FAU - Cornwell, Paul A
AU  - Cornwell PA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cosmet Sci
JT  - Journal of cosmetic science
JID - 9814276
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - DNA/*analysis/genetics
MH  - DNA Primers
MH  - HLA-DQ Antigens/genetics
MH  - HLA-DQ alpha-Chains
MH  - Hair/*chemistry
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - Polymerase Chain Reaction
EDAT- 2003/03/20 04:00
MHDA- 2003/05/09 05:00
CRDT- 2003/03/20 04:00
PHST- 2002/09/06 00:00 [accepted]
PHST- 2003/03/20 04:00 [pubmed]
PHST- 2003/05/09 05:00 [medline]
PHST- 2003/03/20 04:00 [entrez]
PST - ppublish
SO  - J Cosmet Sci. 2003 Jan-Feb;54(1):21-7.