PMID- 12647243
OWN - NLM
STAT- MEDLINE
DCOM- 20031001
LR  - 20061115
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 14
IP  - 3
DP  - 2003 Mar
TI  - Characterization and radiation hybrid mapping of expressed sequence tags from the 
      canine brain.
PG  - 203-13
AB  - Maps of the canine genome are now developing rapidly. Most of the markers on the 
      current integrated canine radiation hybrid/genetic linkage/cytogenetic map are 
      highly polymorphic microsatellite (type II) markers that are very useful for 
      mapping disease loci. However, there is still an urgent need for the mapping of 
      gene-based (type I) markers that are required for comparative mapping, as well as 
      identifying candidate genes for disease loci that have been genetically mapped. 
      We constructed an adult brain cDNA library as a resource to increase the number 
      of gene-based markers on the canine genome map. Eighty-one percent of the 2700 
      sequenced expressed sequence tags (ESTs) represented unique sequences. The canine 
      brain ESTs were compared with sequences in public databases to identify putative 
      canine orthologs of human genes. One hundred nine of the canine ESTs were mapped 
      on the latest canine radiation hybrid (RH) panel to determine the location of the 
      respective canine gene. The addition of these new gene-based markers revealed 
      three conserved segments (CS) between human and canine genomes previously 
      detected by fluorescence in situ hybridization (FISH), but not by RH mapping. In 
      addition, five new CS between dog and human were identified that had not been 
      detected previously by RH mapping or FISH. This work has increased the number of 
      gene-based markers on the canine RH map by approximately 30% and indicates the 
      benefit to be gained by increasing the gene content of the current canine 
      comparative map.
FAU - Roberts, Monica C
AU  - Roberts MC
AD  - College of Veterinary Medicine, Department of Veterinary PathoBiology, University 
      of Minnesota, 1988 Fitch Avenue, 295 Animal Science/Veterinary Medicine, St. Paul 
      55108, USA.
FAU - Hitte, Christophe C
AU  - Hitte CC
FAU - Hendrickson, Julie A
AU  - Hendrickson JA
FAU - Hoffmann, Daniel E
AU  - Hoffmann DE
FAU - Flickinger, Gail H
AU  - Flickinger GH
FAU - Rutherford, Mark S
AU  - Rutherford MS
FAU - Guyon, Richard S
AU  - Guyon RS
FAU - Galibert, Francis S
AU  - Galibert FS
FAU - Mickelson, James R
AU  - Mickelson JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - DNA, Complementary
MH  - Dogs
MH  - *Expressed Sequence Tags
MH  - *Radiation Hybrid Mapping
MH  - Sequence Analysis, DNA
EDAT- 2003/03/21 04:00
MHDA- 2003/10/02 05:00
CRDT- 2003/03/21 04:00
PHST- 2002/06/12 00:00 [received]
PHST- 2002/11/06 00:00 [accepted]
PHST- 2003/03/21 04:00 [pubmed]
PHST- 2003/10/02 05:00 [medline]
PHST- 2003/03/21 04:00 [entrez]
AID - 10.1007/s00335-002-2207-2 [doi]
PST - ppublish
SO  - Mamm Genome. 2003 Mar;14(3):203-13. doi: 10.1007/s00335-002-2207-2.