PMID- 12647244
OWN - NLM
STAT- MEDLINE
DCOM- 20031001
LR  - 20061115
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 14
IP  - 3
DP  - 2003 Mar
TI  - Construction and integration of radiation-hybrid and cytogenetic maps of dog 
      Chromosome X.
PG  - 214-21
AB  - Chromosome (chr) X is under-represented in current maps of the genome of the 
      domestic dog ( Canis familiaris). To address this problem, we have constructed a 
      small-insert, genomic DNA library in pBluescript from flow-sorted canine Chr X 
      DNA. Fluorescence in situ hybridization (FISH) studies confirmed that the library 
      was highly enriched for Chr X. Clones containing microsatellites were identified 
      and sequenced. Database searches detected significant sequence identity between 
      four X-derived clones and genes previously characterized in other species. 
      Thirty-seven markers derived from these clones were mapped on Chr X by FISH, and 
      of these, 28 were mapped by using the female-derived T72 whole-genome radiation 
      hybrid (RH) panel (Research Genetics). Four X-linked canine genes from publicly 
      available data were also mapped. Eight RH linkage groups with LOD >4.0 were 
      identified, and FISH data were used to locate the groups on the chromosome; four 
      groups could be unambiguously orientated by FISH data. In each case, the FISH and 
      RH data were mutually consistent. The data suggest strongly conserved synteny 
      between canine and human X Chrs. The pseudoautosomal region has been further 
      characterized, and the putative or actual locations of nine genes of clinical 
      relevance have been suggested.
FAU - Spriggs, Helen F
AU  - Spriggs HF
AD  - Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU, UK. 
      hfs@mrc-lmb.cam.ac.uk
FAU - Holmes, Nigel G
AU  - Holmes NG
FAU - Breen, Matthew G
AU  - Breen MG
FAU - Deloukas, Panos G
AU  - Deloukas PG
FAU - Langford, Cordelia F
AU  - Langford CF
FAU - Ross, Mark T
AU  - Ross MT
FAU - Carter, Nigel P
AU  - Carter NP
FAU - Davis, Maria E
AU  - Davis ME
FAU - Knights, Catherine E
AU  - Knights CE
FAU - Smith, Angela E
AU  - Smith AE
FAU - Farr, Christine J
AU  - Farr CJ
FAU - McCarthy, Linda C
AU  - McCarthy LC
FAU - Binns, Matthew M
AU  - Binns MM
LA  - eng
SI  - GENBANK/AJ403668
SI  - GENBANK/AJ403705
SI  - GENBANK/AJ405151
SI  - GENBANK/AJ405160
SI  - GENBANK/AJ405162
SI  - GENBANK/AJ405164
SI  - GENBANK/AJ405178
SI  - GENBANK/AJ405185
SI  - GENBANK/AJ405189
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - DNA Primers/metabolism
MH  - Dinucleotide Repeats
MH  - Dogs
MH  - In Situ Hybridization, Fluorescence
MH  - Lod Score
MH  - Molecular Sequence Data
MH  - *Radiation Hybrid Mapping
MH  - Sequence Analysis, DNA
MH  - *X Chromosome
EDAT- 2003/03/21 04:00
MHDA- 2003/10/02 05:00
CRDT- 2003/03/21 04:00
PHST- 2002/08/23 00:00 [received]
PHST- 2002/09/25 00:00 [accepted]
PHST- 2003/03/21 04:00 [pubmed]
PHST- 2003/10/02 05:00 [medline]
PHST- 2003/03/21 04:00 [entrez]
AID - 10.1007/s00335-002-3051-0 [doi]
PST - ppublish
SO  - Mamm Genome. 2003 Mar;14(3):214-21. doi: 10.1007/s00335-002-3051-0.