PMID- 12648063
OWN - NLM
STAT- MEDLINE
DCOM- 20030807
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 120
IP  - 6
DP  - 2003 Mar
TI  - Cytogenetics of multiple myeloma: interpretation of fluorescence in situ 
      hybridization results.
PG  - 944-52
AB  - The cytogenetic picture in multiple myeloma (MM) is highly complex, from which 
      non-random numerical and structural chromosomal changes have been identified. 
      Specifically, translocations involving the immunoglobulin heavy chain gene (IGH) 
      at 14q32 and either monosomy or deletions of chromosome 13 have been reported in 
      a significant number of patients from both cytogenetic and interphase 
      fluorescence in situ hybridization (FISH) studies. Importantly, these 
      abnormalities of chromosome 13 have recently been associated with a poor 
      prognosis. In view of the highly complex nature of the karyotypes in MM patients, 
      interphase FISH results may be difficult to interpret. In this study, 
      cytogenetics and/or interphase FISH were carried out on bone marrow samples or 
      purified plasma cells from 37 MM patients. Abnormal karyotypes, characterized by 
      multiplex FISH (M-FISH) were found in 11 patients, all of which were highly 
      complex. Interphase FISH revealed translocations involving the IGH locus in 16 
      (43%) patients. The IGH/cyclin D1 (CCND1) gene fusion characteristic of the 
      translocation, t(11;14)(q13;q32), was seen in 12 (32%) of these patients and 
      other rearrangements of IGH in four (11%) patients. Fourteen patients had 
      additional copies of chromosome 11. Twenty patients (54%) had 13q14 deletions, 10 
      of whom also had t(11;14) or another IGH translocation. By comparing cytogenetic 
      and FISH results, this study has revealed that significant chromosomal 
      abnormalities might be hidden within highly complex karyotypes. Therefore, 
      extreme caution is required in the interpretation of interphase FISH results in 
      MM, particularly in relation to certain abnormalities, such as 13q14 deletions, 
      which have an impact on prognosis.
FAU - Harrison, Christine J
AU  - Harrison CJ
AD  - Department of Haematology, Royal Free Medical School, London. harrison@soton.acuk
FAU - Mazzullo, Helen
AU  - Mazzullo H
FAU - Cheung, Kan L
AU  - Cheung KL
FAU - Gerrard, Gareth
AU  - Gerrard G
FAU - Jalali, G Reza
AU  - Jalali GR
FAU - Mehta, Atul
AU  - Mehta A
FAU - Osier, David G
AU  - Osier DG
FAU - Orchard, Kim H
AU  - Orchard KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 14
MH  - Cytogenetic Analysis
MH  - Female
MH  - Gene Deletion
MH  - Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - Prognosis
MH  - Translocation, Genetic
EDAT- 2003/03/22 04:00
MHDA- 2003/08/09 05:00
CRDT- 2003/03/22 04:00
PHST- 2003/03/22 04:00 [pubmed]
PHST- 2003/08/09 05:00 [medline]
PHST- 2003/03/22 04:00 [entrez]
AID - 4172 [pii]
AID - 10.1046/j.1365-2141.2003.04172.x [doi]
PST - ppublish
SO  - Br J Haematol. 2003 Mar;120(6):944-52. doi: 10.1046/j.1365-2141.2003.04172.x.