PMID- 12648844
OWN - NLM
STAT- MEDLINE
DCOM- 20030625
LR  - 20181130
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 35
IP  - 3
DP  - 2003 Apr 1
TI  - Production of IL-12 and IL-18 in human dendritic cells upon infection by Listeria 
      monocytogenes.
PG  - 255-62
AB  - Dendritic cells (DCs) are major antigen-presenting cells of the immune system, 
      which need to be activated in order to initiate an immune response. Here, we 
      describe the immunostimulatory effects on human monocyte-derived DCs observed 
      upon infection with Listeria monocytogenes or after treatment with listerial 
      lipoteichoic acid (LTA) and lipopolysaccharide (LPS), respectively. All stimuli 
      caused upregulation of costimulatory molecules, induced T-cell proliferative 
      responses and secretion of cytokines in vitro. Infection of DCs with L. 
      monocytogenes induced release of interleukin (IL)-12 and IL-18. In contrast 
      treatment with purified listerial LTA yielded high levels of IL-18 release, but 
      only minimal IL-12 production. Treatment of DCs with LPS conversely induced 
      significant amounts of IL-12 production, but no IL-18. The release of both 
      stimulating cytokines IL-12 and IL-18 upon infection with entire bacteria 
      suggests that attenuated strains of L. monocytogenes may be a valuable tool for 
      subunit vaccine delivery.
FAU - Kolb-Maurer, Annette
AU  - Kolb-Maurer A
AD  - Department of Dermatology, University of Wurzburg, 97080 Wurzburg, Germany. 
      ankolb@biozentrum.uni-wuerzburg.de
FAU - Kammerer, Ulrike
AU  - Kammerer U
FAU - Maurer, Mathias
AU  - Maurer M
FAU - Gentschev, Ivaylo
AU  - Gentschev I
FAU - Brocker, Eva Bettina
AU  - Brocker EB
FAU - Rieckmann, Peter
AU  - Rieckmann P
FAU - Kampgen, Eckhart
AU  - Kampgen E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Interleukin-18)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Teichoic Acids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Subunit)
RN  - 187348-17-0 (Interleukin-12)
RN  - 56411-57-5 (lipoteichoic acid)
SB  - IM
MH  - Adult
MH  - Bacterial Vaccines
MH  - Cell Differentiation
MH  - Cells, Cultured/metabolism/microbiology
MH  - Dendritic Cells/drug effects/*metabolism/microbiology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interleukin-12/*biosynthesis/genetics
MH  - Interleukin-18/*biosynthesis/genetics
MH  - Lipopolysaccharides/isolation & purification/pharmacology
MH  - Listeria monocytogenes/chemistry/*physiology
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/cytology/drug effects
MH  - Teichoic Acids/isolation & purification/pharmacology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/genetics
MH  - Vaccines, Attenuated
MH  - Vaccines, Subunit
EDAT- 2003/03/22 04:00
MHDA- 2003/06/26 05:00
CRDT- 2003/03/22 04:00
PHST- 2003/03/22 04:00 [pubmed]
PHST- 2003/06/26 05:00 [medline]
PHST- 2003/03/22 04:00 [entrez]
AID - S0928824402004704 [pii]
AID - 10.1016/S0928-8244(02)00470-4 [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2003 Apr 1;35(3):255-62. doi: 
      10.1016/S0928-8244(02)00470-4.