PMID- 12649595
OWN - NLM
STAT- MEDLINE
DCOM- 20031014
LR  - 20190212
IS  - 1015-8987 (Print)
IS  - 1015-8987 (Linking)
VI  - 13
IP  - 2
DP  - 2003
TI  - Cryopreservation of rat precision-cut liver slices is associated with major 
      metabolic stress and ionic perturbations.
PG  - 103-12
AB  - BACKGROUND/AIMS: Cryopreserved precision-cut liver slices (PCLS) in culture 
      exhibit a rapid decline in ATP level and anabolic processes by unknown 
      mechanisms. The aim of this study is to elucidate the key events explaining the 
      alterations occurring in cryopreserved PCLS. METHODS: Glucose metabolism, 
      mitochondrial activities, ionic homeostasis and caspase-3 activity were assessed 
      in fresh or cryopreserved (rapid freezing-thawing conditions) rat PCLS within the 
      first hour of incubation in Williams' medium E at 37 degrees C. RESULTS: Despite 
      a similar glycolytic activity under both conditions, only fresh PCLS were able to 
      gradually recover their ATP and potassium content. Glycogen content dropped more 
      rapidly in cryopreserved than in control PCLS. Only cryopreserved PCLS exhibited 
      a decline in O2 consumption and a lower ATP/ADP ratio from 30 min of incubation 
      with a loss of coupling of oxygen consumption to ATP synthesis and mitochondrial 
      calcium transport after 1 hour of incubation. Caspase-3 activation was already 
      present in cryopreserved PCLS after a few minutes of incubation. CONCLUSIONS: The 
      lack of restoration of potassium/sodium exchange might be a primary event in the 
      metabolic alterations occurring in cryopreserved PCLS after thawing, that 
      contributes to further mitochondrial alterations, caspase activation and 
      compromises ATP-dependent anabolic pathways.
FAU - Vanhulle, Valerie P
AU  - Vanhulle VP
AD  - Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology, Universite 
      Catholique de Louvain, Belgium.
FAU - Martiat, Genevieve A
AU  - Martiat GA
FAU - Bontemps, Francoise
AU  - Bontemps F
FAU - Vincent, Marie-Francoise
AU  - Vincent MF
FAU - Pycke, Jean-Marie
AU  - Pycke JM
FAU - Verbeeck, Roger K
AU  - Verbeeck RK
FAU - Horsmans, Yves
AU  - Horsmans Y
FAU - Delzenne, Nathalie
AU  - Delzenne N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Ions)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - *Cryopreservation
MH  - Freezing
MH  - Glucose/metabolism
MH  - Ions/metabolism
MH  - Lipid Metabolism
MH  - Liver/*metabolism
MH  - Male
MH  - Mitochondria/metabolism
MH  - Oxygen Consumption
MH  - Proton-Translocating ATPases/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Stress, Physiological/*metabolism
MH  - Time
EDAT- 2003/03/22 04:00
MHDA- 2003/10/15 05:00
CRDT- 2003/03/22 04:00
PHST- 2002/11/05 00:00 [accepted]
PHST- 2003/03/22 04:00 [pubmed]
PHST- 2003/10/15 05:00 [medline]
PHST- 2003/03/22 04:00 [entrez]
AID - 70254 [pii]
AID - 10.1159/000070254 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2003;13(2):103-12. doi: 10.1159/000070254.