PMID- 12651914 OWN - NLM STAT- MEDLINE DCOM- 20040819 LR - 20181113 IS - 0022-3751 (Print) IS - 1469-7793 (Electronic) IS - 0022-3751 (Linking) VI - 548 IP - Pt 3 DP - 2003 May 1 TI - Skeletal muscle fat and carbohydrate metabolism during recovery from glycogen-depleting exercise in humans. PG - 919-27 AB - The primary aim of the present study was to determine whether intramuscular triacylglycerol (IMTG) utilization contributed significantly to the increase in lipid oxidation during recovery from exercise, as determined from the muscle biopsy technique. In addition, we also examined the regulation of pyruvate dehydrogenase (PDHa) and changes in muscle acetyl units during an 18 h recovery period after glycogen-depleting exercise. Eight endurance-trained males completed an exhaustive bout of exercise (approximately 90 min) on a cycle ergometer followed by ingestion of carbohydrate (CHO)-rich meals (64-70 % of energy from carbohydrate) at 1, 4 and 7 h of recovery. Duplicate muscle biopsies were obtained at exhaustion, and 3, 6 and 18 h of recovery. Despite the large intake of CHO during recovery (491 +/- 28 g or 6.8 +/- 0.3 g kg-1), respiratory exchange ratio values of 0.77 to 0.84 indicated a greater reliance on lipid as an oxidative fuel. However, there was no net IMTG utilization during recovery. IMTG content at exhaustion was 23.5 +/- 3.5 mmol (kg dry wt)-1, and remained constant at 24.6 +/- 2.6, 25.7 +/- 2.8 and 28.4 +/- 3.0 mmol (kg dry wt)-1 after 3, 6 and 18 h of recovery. Muscle glycogen increased significantly from 37 +/- 11 mmol (kg dry wt)-1 at exhaustion, to 165 +/- 13, 250 +/- 18, and 424 +/- 22 mmol (kg dry wt)-1 at 3, 6 and 18 h of recovery, respectively. PDHa was reduced at 6 and 18 h when compared to exhaustion, but did not change during the recovery period. Acetyl-CoA, acetylcarnitine and pyruvate contents declined significantly after 3 h of recovery compared to exhaustion, and thereafter remained unchanged. We conclude that IMTG has a negligible role in contributing to the enhanced fat oxidation during recovery from exhaustive exercise. Despite the elevation of glucose and insulin following high-CHO meals during recovery, CHO oxidation and PDH activation were decreased, supporting the hypothesis that glycogen resynthesis is of high metabolic priority. Plasma fatty acids, very low density lipoprotein triacylglycerols, as well as intramuscular acetylcarnitine stores are likely to be important fuel sources for aerobic energy, particularly during the first few hours of recovery. FAU - Kimber, Nicholas E AU - Kimber NE AD - Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada. FAU - Heigenhauser, George J F AU - Heigenhauser GJ FAU - Spriet, Lawrence L AU - Spriet LL FAU - Dyck, David J AU - Dyck DJ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20030321 PL - England TA - J Physiol JT - The Journal of physiology JID - 0266262 RN - 0 (Blood Glucose) RN - 0 (Pyruvate Dehydrogenase Complex) RN - 0 (Triglycerides) RN - 9005-79-2 (Glycogen) SB - IM MH - Adult MH - Basal Metabolism MH - Blood Glucose/metabolism MH - *Carbohydrate Metabolism MH - Eating MH - Energy Intake MH - Exercise/*physiology MH - Fasting MH - Glycogen/deficiency/*metabolism MH - Humans MH - Lipid Metabolism MH - Male MH - Muscle, Skeletal/*physiology MH - Oxygen Consumption MH - Physical Endurance/*physiology MH - Postprandial Period MH - Pyruvate Dehydrogenase Complex/metabolism MH - Respiratory Mechanics MH - Sports MH - Triglycerides/*metabolism PMC - PMC2342904 EDAT- 2003/03/26 04:00 MHDA- 2004/08/20 05:00 PMCR- 2004/05/01 CRDT- 2003/03/26 04:00 PHST- 2003/03/26 04:00 [pubmed] PHST- 2004/08/20 05:00 [medline] PHST- 2003/03/26 04:00 [entrez] PHST- 2004/05/01 00:00 [pmc-release] AID - 2002.031179 [pii] AID - 10.1113/jphysiol.2002.031179 [doi] PST - ppublish SO - J Physiol. 2003 May 1;548(Pt 3):919-27. doi: 10.1113/jphysiol.2002.031179. Epub 2003 Mar 21.