PMID- 12652294
OWN - NLM
STAT- MEDLINE
DCOM- 20030530
LR  - 20220225
IS  - 1078-8956 (Print)
IS  - 1078-8956 (Linking)
VI  - 9
IP  - 4
DP  - 2003 Apr
TI  - Detection of virus-specific T cells and CD8+ T-cell epitopes by acquisition of 
      peptide-HLA-GFP complexes: analysis of T-cell phenotype and function in chronic 
      viral infections.
PG  - 469-76
AB  - Antigen-specific CD8+ T cells acquire peptide-major histocompatibility complex 
      (MHC) clusters through T-cell receptor (TCR)-mediated endocytosis after specific 
      antigen stimulation. We generated an antigen-presenting cell (APC) expressing 
      human leukocyte antigen (HLA)-A*201 coupled to the enhanced green fluorescent 
      protein (GFP), which delivered GFP to an antigen-specific T cell when pulsed with 
      antigenic peptide. We quantitatively identified human T-cell lymphotropic virus 
      type I (HTLV-I) Tax(11-19) peptide-specific T-cell populations in peripheral 
      blood mononuclear cells (PBMCs) from patients with HTLV-I-associated neurologic 
      disease and defined a new CD8+ T-cell epitope in the HTLV-I envelope region. 
      Acquisition of peptide-HLA-GFP complexes by antigen-specific T cells could 
      distinguish, with respect to phenotype and perforin production, T cells from the 
      chronic viral infections cytomegalovirus and HTLV-I. This approach will be a 
      powerful tool in understanding the role of antigen-specific T-cell responses in 
      health and disease.
FAU - Tomaru, Utano
AU  - Tomaru U
AD  - Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, 
      National Institutes of Health, Bethesda, Maryland, USA.
FAU - Yamano, Yoshihisa
AU  - Yamano Y
FAU - Nagai, Masahiro
AU  - Nagai M
FAU - Maric, Dragan
AU  - Maric D
FAU - Kaumaya, Previn T P
AU  - Kaumaya PT
FAU - Biddison, William
AU  - Biddison W
FAU - Jacobson, Steven
AU  - Jacobson S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030324
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - 0 (Carrier Proteins)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (TAX1BP1 protein, human)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Antigen-Presenting Cells/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Carrier Proteins/analysis
MH  - Chronic Disease
MH  - Cytomegalovirus Infections/immunology
MH  - Green Fluorescent Proteins
MH  - HLA-A Antigens/*metabolism
MH  - Humans
MH  - Immunodominant Epitopes
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Luminescent Proteins/*metabolism
MH  - Neoplasm Proteins/analysis
MH  - Paraparesis, Tropical Spastic/immunology
MH  - Phenotype
MH  - T-Lymphocytes/*immunology
MH  - Virus Diseases/*immunology
EDAT- 2003/03/26 04:00
MHDA- 2003/05/31 05:00
CRDT- 2003/03/26 04:00
PHST- 2003/03/26 04:00 [pubmed]
PHST- 2003/05/31 05:00 [medline]
PHST- 2003/03/26 04:00 [entrez]
AID - nm845 [pii]
AID - 10.1038/nm845 [doi]
PST - ppublish
SO  - Nat Med. 2003 Apr;9(4):469-76. doi: 10.1038/nm845. Epub 2003 Mar 24.