PMID- 12655031
OWN - NLM
STAT- MEDLINE
DCOM- 20030926
LR  - 20221207
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 72
IP  - 2
DP  - 2003 Apr
TI  - Gestational exposure to persistent organic pollutants: maternal liver residues, 
      pregnancy outcome, and effects on hepatic gene expression profiles in the dam and 
      fetus.
PG  - 242-52
AB  - Dietary exposure of Inuit people to a mixture of pesticides and polychlorinated 
      biphenyls, or persistent organic pollutants (POPs), during pregnancy is a public 
      health concern. We examined the consequences of administering the mixture of 28 
      POPs found in the Inuit diet (at doses representing 10-1000 times dietary levels) 
      by gavage to pregnant Sprague-Dawley rats either during gestation days 0-19 or 
      8-19. The levels of individual components of the POPs mixture in the maternal 
      liver were measured by high-resolution mass spectrometry. On gestation day 20, 
      dams were sacrificed and pregnancy outcome determined. RNA isolated from maternal 
      and fetal livers was 32P-labeled for gene expression profiling. The 
      concentrations of individual POPs were increased in maternal livers of dams 
      gavaged with the 1000x POPs mixture by 10- to 500-fold. While exposure to POPs 
      had no significant effects on pregnancy outcome, dramatic changes were observed 
      in the gene expression profiles of both the maternal and fetal livers. The gene 
      expression profiles of maternal and fetal female and male liver were distinct 
      with respect to the numbers of transcripts detected, the genes expressed 
      exclusively in control or POPs-exposed livers, and those for which expression was 
      up- or downregulated. While different genes were affected in each group, the 
      overall consequence of POPs exposure on hepatic gene expression profiles was to 
      decrease both the numbers of genes expressed and the relative intensity of 
      expression. Thus, in utero exposure to POPs alters hepatic gene expression in the 
      dam and the fetus; these changes may have functional implications.
FAU - Adeeko, Adedayo
AU  - Adeeko A
AD  - Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade 
      Sir-William-Osler, Montreal, Quebec H3G 1Y6, Canada.
FAU - Li, Daming
AU  - Li D
FAU - Doucet, Josee
AU  - Doucet J
FAU - Cooke, Gerard M
AU  - Cooke GM
FAU - Trasler, Jacquetta M
AU  - Trasler JM
FAU - Robaire, Bernard
AU  - Robaire B
FAU - Hales, Barbara F
AU  - Hales BF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030307
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Environmental Pollutants)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Insecticides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Diet
MH  - Environmental Pollutants/administration & dosage/*toxicity
MH  - Female
MH  - Fetus/*drug effects/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - *Hydrocarbons, Chlorinated
MH  - Insecticides/administration & dosage/*toxicity
MH  - *Inuit
MH  - Liver/*drug effects/embryology/metabolism
MH  - Male
MH  - Pregnancy
MH  - Pregnancy, Animal/*drug effects
MH  - RNA, Messenger/analysis/metabolism
MH  - Rats
EDAT- 2003/03/26 05:00
MHDA- 2003/09/27 05:00
CRDT- 2003/03/26 05:00
PHST- 2003/03/26 05:00 [pubmed]
PHST- 2003/09/27 05:00 [medline]
PHST- 2003/03/26 05:00 [entrez]
AID - kfg023 [pii]
AID - 10.1093/toxsci/kfg023 [doi]
PST - ppublish
SO  - Toxicol Sci. 2003 Apr;72(2):242-52. doi: 10.1093/toxsci/kfg023. Epub 2003 Mar 7.