PMID- 12656215
OWN - NLM
STAT- MEDLINE
DCOM- 20030716
LR  - 20190901
IS  - 0301-4800 (Print)
IS  - 0301-4800 (Linking)
VI  - 48
IP  - 5
DP  - 2002 Oct
TI  - Digestibility and chemical species of selenium contained in high-selenium yeast.
PG  - 401-4
AB  - To evaluate the bioavailability of selenium (Se) in high-Se yeast (SeY), the 
      digestibility and chemical species of Se in SeY were investigated. Both Se and 
      nitrogen in SeY were readily released into the soluble fraction through trypsin 
      digestion. In a Sephadex G-25 gel chromatography of the trypsin digest of SeY, 
      the range in which Se was eluted was coincident with the range in which peptide 
      fragments were eluted. Se was distributed almost uniformly within the range and 
      there was no fraction that contained Se in a specifically high amount. A 
      proteolytic enzyme extract of SeY was found to contain Se as selenomethionine 
      (74.8%), selenocystine (9.9%), selenite (5.1%) and as at least three unknown Se 
      compounds (10.2%) when analyzed using high-performance liquid 
      chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS). These 
      results indicate that Se in SeY is mainly present as selenomethionine 
      non-specifically incorporated into peptide chains and is highly digestible. 
      Accordingly, it is concluded that the bioavailability of Se in SeY is high.
FAU - Yoshida, Munehiro
AU  - Yoshida M
AD  - Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty 
      of Engineering, Kansai University, Suita, Osaka 564-8680, Japan. 
      hanmyou4@ipcku.kansai-u.ac.jp
FAU - Sugihara, Satoru
AU  - Sugihara S
FAU - Suenaga, Toru
AU  - Suenaga T
FAU - Naito, Chitose
AU  - Naito C
FAU - Fukunaga, Kenji
AU  - Fukunaga K
FAU - Tsuchita, Hiroshi
AU  - Tsuchita H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Nutr Sci Vitaminol (Tokyo)
JT  - Journal of nutritional science and vitaminology
JID - 0402640
RN  - EC 3.4.21.4 (Trypsin)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Digestion/physiology
MH  - In Vitro Techniques
MH  - Mass Spectrometry
MH  - Saccharomyces cerevisiae/*chemistry
MH  - Selenium/*chemistry/*metabolism
MH  - Trypsin/pharmacology
EDAT- 2003/03/27 05:00
MHDA- 2003/07/17 05:00
CRDT- 2003/03/27 05:00
PHST- 2003/03/27 05:00 [pubmed]
PHST- 2003/07/17 05:00 [medline]
PHST- 2003/03/27 05:00 [entrez]
AID - 10.3177/jnsv.48.401 [doi]
PST - ppublish
SO  - J Nutr Sci Vitaminol (Tokyo). 2002 Oct;48(5):401-4. doi: 10.3177/jnsv.48.401.